ENGLISH

Bacterial Pathogenesis, Part A Identification and Regulation of Virulence Factors

Book information

Publisher
Academic Press
Year
1994
ISBN
0121821366, 9780121821364
ISSN
0076-6879
Google Books ID
BdbwAAAAMAAJ
Open Library ID
OL10071270M
Language
english
Format
PDF
Filesize
59 MB (61912945 bytes)
Series
Methods In Enzymology 235
Edition
1
Pages
826\826
Orientation
yes
Scanned
yes
Time added
2012-02-04 16:00:00

Description

This volume of Methods in Enzymology presents methods for the isolation and identification of bacterial pathogens and associated virulence determinants. It will enable the researcher to determine if closed genes or isolated gene products are involved in virulence. Major Topics Covered Include* Animal model system to determine bacterial virulence* Epidemiological techniques for the identification of bacterial strains and species* Protocols for the purification of subcellular bacterial products frequently associated with virulence* Determination of the means by which bacteria acquire iron* Methods to generate mutants, to construct isogenic strains by allelic exchange, to identify bacterial genes only expressed during infection of the host, and to study regulation of selected bacterial virulence factors* Methods for the assay of destructive bacterial enzymes and toxins

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