ENGLISH

MAIT Cells: Methods and Protocols

By Helen Kaipe; Isabelle Magalhaes

Book information

Publisher
Human
Year
2020
ISBN
1071602071, 9781071602072
Language
english
Format
EPUB
Filesize
28 MB (29372703 bytes)
Pages
310\0
Time added
2020-06-21 07:27:27

Description

This volume focuses on various methods used by researchers to study mucosal-associated invariant T (MAIT) cells in the aspects described below. The chapters are organized into Four Parts: part One looks at the function and importance of MAIT cells in health and disease. It covers methods to isolate and characterize MAIT cells from human tissues including liver, colon tumors, placenta and decidua, and endometrium. Part Two discusses MR1 independent stimulation, and looks at the activation of MAIT cells by different stimulatory agents. Part Three describes various methods to develop tools to analyze, monitor, and generate MAIT cells in vitro, including the production of MR1-tetramers and how they can be loaded with bacterial antigens and used for the detection of MAIT cells. The last part covers the use of murine models to study MAIT cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, MAIT Cells: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing field. Chapters 9 and 17 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

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