ENGLISH

Two-Hybrid Systems: Methods and Protocols

By Paul N. MacDonald

Book information

Publisher
Humana Press
Year
2001
ISBN
9780896038080, 0-89603-808-4, 0-89603-832-7
Open Library ID
OL8253730M
Language
english
Format
PDF
Filesize
2 MB (1934602 bytes)
Series
Methods in Molecular Biology
Edition
1
Pages
336\336
Topic
Biology
Time added
2009-10-15 16:43:54

Description

Paul N. MacDonald has assembled a collection of powerful molecular tools for examining and characterizing protein-protein, protein-DNA, and protein-RNA interactions. The techniques range from the most basic (introducing plasmids into yeasts, interaction assays, and recovering the plasmids from yeast), to the most advanced alternative strategies (involving one-hybrid, split two-hybrid, three-hybrid, membrane recruitment systems, and mammalian systems). Methods are also provided for dealing with the well-known problem of artifacts and false positives and for identifying the interacting partners in important biological systems, including the SMAD and nuclear receptor pathways. To ensure ready reproducibility and robust results, each technique is described in step-by-step detail by researchers who employ it regularly.

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