ENGLISH

CRISPR Guide RNA Design: Methods and Protocols

By Tudor A. Fulga , David J. H. F. Knapp , Quentin R. V. Ferry

Book information

Publisher
Springer US;Humana
Year
2021
ISBN
9781071606865, 9781071606872
DOI
10.1007/978-1-0716-0687-2
Language
english
Format
PDF
Filesize
10 MB (10140983 bytes)
Series
Methods in Molecular Biology 2162
Edition
1st ed.
Pages
X, 286\287
Time added
2021-01-06 05:41:07
Life Sciences; Genetics and Genomics; Biomedical E

Description

This detailed volume focuses on the CRISPR-associated guide RNA and how it can be designed, modified, and validated for a broad repertoire of purposes. Beginning with a section on computational design of target-specific guide RNAs, the book continues by covering chemical modifications to alter guide RNA stability, specificity, and efficiency, as well as to create inducible guide RNAs, append additional functional domains, and express guide RNAs in a conditional manner. It concludes with methods for measuring off-target guide RNA activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and essential, CRISPR Guide RNA Design: Methods and Protocols provides a comprehensive pipeline for guide RNA design and aims to be an invaluable resource in applying this powerful technology to basic research and therapeutic applications.

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