ENGLISH

Mass Spectrometry of Glycoproteins: Methods and Protocols

By Steven M. Patrie , Michael J. Roth , Jennifer J. Kohler (auth.) , Jennifer J. Kohler , Steven M. Patrie (eds.)

Book information

Publisher
Humana Press
Year
2013
ISBN
1627031456, 9781627031455
DOI
10.1007/978-1-62703-146-2
ISSN
1064-3745
LCC
2012950354
Language
english
Format
PDF
Filesize
6 MB (6126687 bytes)
Series
Methods in Molecular Biology 951
Edition
1
Pages
339\353
Topic
Biology Molecular
Orientation
yes
Paginated
yes
Scanned
portrait
Time added
2013-08-07 17:58:44
Protein Science; Mass Spectrometry

Description

Glycosylation is the most abundant post-translational modification of proteins. Estimates vary widely, but a common assessment is that upwards of 50% of eukaryotic proteins are modified by some type of glycan. In Mass Spectrometry of Glycoproteins: Methods and Protocols, expert researchers in the field detail many of the methods that are now commonly used for glycoproteomics. These methods and techniques include robust sample preparation techniques; advanced chromatographic strategies for improving dynamic range; state-of-the-art mass spectrometry instrumentation and associated ionization and fragmentation methods; and informatics tools used for identifying glycoproteins and characterizing the associated glycans. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mass Spectrometry of Glycoproteins: Methods and Protocol is an essential resource for those who work at the interface of glycobiology and mass spectrometry.

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