Replacing Animal Models: A Practical Guide to Creating and Using Culture-Based Biomimetic Alternatives

Description

Over the last decade, in vitro models have become more sophisticated and are at a stage where they can provide an effective alternative to in vivo experiments. Replacing Animal Models provides scientists and technicians with a practical, integrated guide to developing culture-based alternatives to in vivo experiments. The book is neither political nor polemical: it is technical, illustrating by example how alternatives can be developed and used and providing useful advice on developing others. After looking at the reasons for and potential benefits of alternatives to animal experiments, the book covers a range of methods and examples emphasising the design considerations that went into each system. The chapters also include 'case studies' that illustrate the ways in which culture models can be used to answer a range of important biological questions of direct relevance to human development, physiology, disease and healing. The thesis of this book is not that all animal experimentation can be replaced, now or in the near future, by equally effective or superior alternatives. Rather, the premise is that there is substantial opportunity, here and now, to do some common types of experiment better in vitro than in vivo, and that doing so will result in both scientific and ethical gains.Content: Chapter 1 Potential Advantages of Using Biomimetic Alternatives (pages 1–11): Jamie DaviesChapter 2 Overview of Biomimetic Alternatives (pages 13–19): Jamie DaviesChapter 3 Pancreatic Islets (pages 21–33): Eli C LewisChapter 4 Endometrial Organoid Culture (pages 35–44): Merja BlauerChapter 5 Modelling Lymphatic and Blood Capillary Patterning (pages 45–56): Francoise Bruyere, Catherine Maillard, Charlotte Erpicum and Agnes NoelChapter 6 Precision?Cut Lung Slices (PCLS) (pages 57–67): Christian Martin and Stefan UhligChapter 7 Human Colon Tissue in Organ Culture (pages 69–80): Michael K Dame and James VaraniChapter 8 Fetal Organ Culture (pages 81–87): Jamie DaviesChapter 9 Design of a Mechanical Loading Device to Culture Intact Bovine Spinal Motion Segments under Multiaxial Motion (pages 89–105): Jochen Walser, Stephen John Ferguson and Benjamin Gantenbein?RitterChapter 10 Magnetic Assembly of Tissue Surrogates (pages 107–114): Chien?Yu Fu and Hwan?You ChangChapter 11 Assembly of Renal Tissues by Cellular Self?Organization (pages 115–122): Mathieu UnbekandtChapter 12 Hierarchical Screening of Pathways: Using Cell and Organ Cultures to Reduce use of Transgenic Mice (pages 123–136): Guangping Tai and Jamie DaviesChapter 13 Lung Organoid Culture to Study Responses to Viruses (pages 137–149): Wenxin Wu, J Leland Booth and Jordan P MetcalfChapter 14 Organ?Cultured Human Skin for the Study of Epithelial Cell Invasion of Stroma (pages 151–158): James VaraniChapter 15 Organotypic Mandibular Cultures for the Study of Inflammatory Bone Pathology (pages 159–166): Alastair J Sloan, Sarah Y Taylor and Emma L SmithChapter 16 Three?Dimensional, High?Density and Tissue Engineered Culture Models of Articular Cartilage (pages 167–192): Ali Mobasheri, Sara Kelly, Abigail L. Clutterbuck, Constanze Buhrmann and Mehdi ShakibaeiChapter 17 Concluding Remarks (pages 193–194): Jamie Davies