Molecular Analyses

Description

DNA and RNA extraction methods from a variety of tissues and samples are now routine, including extraction from single cells. Many methods are now automated. Sequencing efficiency has reached the point where it is now possible to obtain gigabases of data, both quickly and inexpensively. Such methods permit the identification of gene versions, including those associated with disease (e.g. small nucleotide polymorphism analyses, or SNPs). The general public as well as clinicians can now access a wide variety of literature on the molecular bases of diseases, allowing them to better assess disease risks and treatments. This volume concentrates on medically-focused methods, and therefore the major audience will be medical professionals, students, and those involved in medically-related research endeavors. There are also papers in this volume dealing specifically with methods developed to analyze large sequence data sets. Many methods reviewed herein are more broadly applicable to other fields in biology, chemistry, bioinformatics, and bioengineering, and are intended for a broad readership. Key Features Summarizes nucleic acid extractions from a wide variety of tissues and cells Describes processes of nucleic acid preservation Reviews forensic sampling, detection of nucleic acids, and delivery of nucleic acids to multicellular organisms Provides essential guidance for sequencing, sequence analysis, database searches, and phylogenetic analyses Includes additional methods useful for analysis of nucleic acids and proteins Related Titles DeSalle, et al. Phylogenomics: A Primer (ISBN 978-0-3670-2849-7). Jennings, W. B. Phylogenomic Data Acquisition: Principles and Practice (ISBN 978-0-3678-6980-9). Wang, X. Next-Generation Sequencing Data Analysis (ISBN 978-1-4822-1788-9) Sung, W.-K. Algorithms for Next-Generation Sequencing (ISBN 978-0-3676-5797-0) Cover Half Title Series Page Title Page Copyright Page Contents Preface List of Contributors Chapter 1: Nucleic Acid Extraction from Diverse Samples Chapter 2: DNA Extraction from Mummified Tissues Chapter 3: Commercial DNA Extraction Kits Chapter 4: Automated Nucleic Acid Extraction Chapter 5: Forensic DNA Samples: Collection and Handling Chapter 6: DNA Preservation Chapter 7: RNA Storage Chapter 8: Disposable Electrochemical DNA Biosensors Chapter 9: Handheld Nucleic Acid Analyzer Chapter 10: DNA-Binding Fluorophores Chapter 11: Fluorescence Resonance Energy Transfer (FRET) Chapter 12: LightUp® Probes Chapter 13: Invader® Assay Chapter 14: Laser-Capture Microdissection Chapter 15: Helper-Dependent Adenoviral Vectors Chapter 16: Retroviral Vectors Chapter 17: Liposomal Nonviral Delivery Vehicles Chapter 18: Polymer-Based Nonviral Nucleic Acid Delivery and Genome Editing Chapter 19: Automated DNA Sequencing Chapter 20: DNA Sequencing Methods Chapter 21: Differential Sequencing by Mass Spectrometry Chapter 22: Brief Guide to Conducting Biological Database Searches Chapter 23: Accessing Genomic Databases Chapter 24: Phylogenetics, Comparative Genomics, and Phylogenomics Chapter 25: DNannotator: Annotation Software Tool Kit for Regional Genomic Sequences Chapter 26: ESTAnnotator: A Tool for High-Throughput EST Annotation Chapter 27: The Basics of Omics Chapter 28: Ribotyping Chapter 29: Forensic DNA Typing: Y Chromosome Chapter 30: Forensic Identification: An Overview on Molecular Diagnostic Technology Chapter 31: Denaturing Gradient Gel Electrophoresis (DGGE) Chapter 32: Heteroduplex Analysis (HA) Chapter 33: NEBcutter: A Program to Cleave DNA with Restriction Enzymes Chapter 34: Differential Display (DD) Analysis Chapter 35: Protein Truncation Test (PTT) Index