Host-Pathogen Interactions: Methods and Protocols

Description

This detailed volume explores numerous multidisciplinary approaches employed to analyze the role of different molecules or strategies used by different guests to survive inside hosts. Divided into several sections, the book delves into animal-pathogen interaction, microbe-microbe interaction, as well as plant-pathogen interaction. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Host-Pathogen Interactions: Methods and Protocols provides many molecular and bioinformatics techniques currently in use at reputed laboratories in countries all around the world, vital for many kinds of host-pathogen interactions studies. Front Matter ....Pages i-xvi Genetic Association Studies in Host–Pathogen Interaction Analysis (Jose Luis Royo, Luis Miguel Real)....Pages 1-11 Bacterial Genotyping Methods: From the Basics to Modern (Margarita M. Ochoa-Díaz, Silvana Daza-Giovannetty, Doris Gómez-Camargo)....Pages 13-20 Front Matter ....Pages 21-21 Real-Time Reverse Transcription PCR as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens (Gili Aviv, Ohad Gal-Mor)....Pages 23-32 Usage of a Bioluminescence Reporter System to Image Promoter Activity During Host Infection (Gili Aviv, Ohad Gal-Mor)....Pages 33-38 lacZ Reporter System as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens (Gili Aviv, Ohad Gal-Mor)....Pages 39-45 Western Blotting Against Tagged Virulence Determinants to Study Bacterial Pathogenicity (Gili Aviv, Ohad Gal-Mor)....Pages 47-54 Molecular Methods to Analyze the Effect of Proteins Expressed by Salmonella During Its Intracellular Stage (Carlos Medina, Beatriz Mesa-Pereira, Eva M. Camacho, Amando Flores, Eduardo Santero)....Pages 55-70 Organoids as a Model to Study Infectious Disease (Kristen A. Engevik, Andrea L. Matthis, Marshall H. Montrose, Eitaro Aihara)....Pages 71-81 Surface Proteome Biotinylation Combined with Bioinformatic Tools as a Strategy for Predicting Pathogen Interacting Proteins (Anita Horvatić, Josipa Kuleš, Nicolas Guillemin, Franjo Martinković, Iva Štimac, Vladimir Mrljak et al.)....Pages 83-96 Systems Biology Modeling to Study Pathogen–Host Interactions (Müberra Fatma Cesur, Saliha Durmuş)....Pages 97-112 Phage Therapy: Various Perspectives on How to Improve the Art (Stephen T. Abedon)....Pages 113-127 Front Matter ....Pages 129-129 Application of RNA-seq and Bioimaging Methods to Study Microbe–Microbe Interactions and Their Effects on Biofilm Formation and Gene Expression (Cristina Isabel Amador, Claus Sternberg, Lars Jelsbak)....Pages 131-158 Serial Dilution-Based Growth Curves and Growth Curve Synchronization for High-Resolution Time Series of Bacterial Biofilm Growth (Fernando Govantes)....Pages 159-169 Detection of Bacterial Quorum Sensing Molecules (Elke Stein, Adam Schikora)....Pages 171-179 Front Matter ....Pages 181-181 Generating Chromosome-Located Transcriptional Fusions to Fluorescent Proteins for Single-Cell Gene Expression Analysis in Pseudomonas syringae (José S. Rufián, Diego López-Márquez, Nieves López-Pagán, Murray Grant, Javier Ruiz-Albert, Carmen R. Beuzón)....Pages 183-199 Introduction of Genetic Material in Ralstonia solanacearum Through Natural Transformation and Conjugation (Anthony Perrier, Patrick Barberis, Stéphane Genin)....Pages 201-207 In Vitro and In Vivo Secretion/Translocation Assays to Identify Novel Ralstonia solanacearum Type 3 Effectors (Fabien Lonjon, Nemo Peeters, Stéphane Genin, Fabienne Vailleau)....Pages 209-222 Plant Pathogenicity Phenotyping of Ralstonia solanacearum Strains (Arry Morel, Nemo Peeters, Fabienne Vailleau, Patrick Barberis, Gaofei Jiang, Richard Berthomé et al.)....Pages 223-239 Methods to Quantify Biotic-Induced Stress in Plants (Marcel Bach-Pages, Gail M. Preston)....Pages 241-255 From Sample to Data: Preparing, Obtaining, and Analyzing Images of Plant-Pathogen Interactions Using Confocal Microscopy (Helen N. Fones, George R. Littlejohn)....Pages 257-262 Screening of c-di-GMP-Regulated Exopolysaccharides in Host Interacting Bacteria (Jochen Schmid, Broder Rühmann, Volker Sieber, Lorena Romero-Jiménez, Juan Sanjuán, Daniel Pérez-Mendoza)....Pages 263-275 Primary Characterization of Small RNAs in Symbiotic Nitrogen-Fixing Bacteria (Marta Robledo, Natalia I. García-Tomsig, José I. Jiménez-Zurdo)....Pages 277-295 A New, Nondestructive, Split-Root System for Local and Systemic Plant Responses Studies with Soybean (Ángeles Hidalgo, José E. Ruiz-Sainz, José M. Vinardell)....Pages 297-306 Methods for the Characterization of Plant-Growth Promoting Rhizobacteria (Md. Motaher Hossain, Farjana Sultana)....Pages 307-328 Back Matter ....Pages 329-332