ENGLISH

Methods in Molecular Biology Volume 176: Steroid Receptor Methods; Protocols and Assays

By Benjamin A. Lieberman

Book information

Publisher
Humana Press
Year
2001
ISBN
0896037541
Language
english
Format
PDF
Filesize
2 MB (1689540 bytes)
Edition
1st
Pages
397\397
Time added
2011-08-31 04:54:40

Description

A distinguished team of principal investigators and their associates describe in step-by-step detail a cross-section of the latest research techniques available for studying the endocrine system. As a basis for sophisticated biochemical analysis of receptor properties, the contributors provide methods for the production and purification of a variety of receptors, including progesterone, glucocorticoid, and androgen. Other protocols allow the reader to experiment with DNA binding characteristics, hormone binding assays, and the use of combinatorial chemistry for drug discovery. A series of novel methods utilizing the latest advances in immunochemistry, yeast two-hybrid screening, and fluorescence are included for the detection and analysis of a variety of cellular proteins that influence steroid receptor effectiveness.

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