Co-Immunoprecipitation Methods for Brain Tissue

Description

This volume covers various aspects of co-immunoprecipitation (co-IP) methods and its relevant use in studying protein-protein interactions (PPIs) in health and diseases of the Central Nervous System. The chapters in this book discuss topics such as using co-IP to detect G protein-coupled receptors (GPCR), receptor tyrosine kinases (RTK) and ion channels heteroreceptor complexes in brain tissue; the histoblot technique; interaction strength between synaptic proteins using COS-7; and co-IP analysis of the protein-protein interactions in the neurons of Polymita. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and thorough, Co-Immunoprecipitation Methods for Brain Tissue is a valuable resource for any researcher interesting in learning more about this developing field. Front Matter ....Pages i-xiii Co-immunoprecipitation Methods for Detection of G Protein-Coupled Receptors in Brain Tissue (Kazunori Namba, Hiroki Kaneko)....Pages 1-8 Co-immunoprecipitation Methods to Identify Associated Proteins with Estrogen Receptor α at Postsynaptic Density in Brain Tissue (Gen Murakami, Suguru Kawato)....Pages 9-21 The Use of Co-immunoprecipitation to Study Conformation-Specific Protein Interactions of Oligomeric α-Synuclein Aggregates (Cristine Betzer, Rikke Hahn Kofoed, Poul Henning Jensen)....Pages 23-36 Using Co-immunoprecipitation and Shotgun Mass Spectrometry for Protein-Protein Interaction Identification in Cultured Human Oligodendrocytes (Bradley Smith, Daniel Martins-de-Souza, Mariana Fioramonte)....Pages 37-47 Co-immunoprecipitation Analysis of GPCR Complexes in the Central Nervous System (Yuji Kamikubo, Takashi Sakurai)....Pages 49-64 The Histoblot Technique: A Reliable Approach to Analyze Expression Profile of Proteins and to Predict Their Molecular Association (Carolina Aguado, Rafael Luján)....Pages 65-88 Co-immunoprecipitation Assay to Investigate the Interaction Strength Between Synaptic Proteins Using COS-7 Cells (Sosuke Yagishita)....Pages 89-96 Guanosine-5′-O-(3-[35S]thio)triphosphate ([35S]GTPγS) Binding/Immunoprecipitation Assay Using Magnetic Beads Coated with Anti-Gα Antibody in Mammalian Brain Membranes (Yuji Odagaki)....Pages 97-107 Co-immunoprecipitation as a Useful Tool for Detection of G Protein-Coupled Receptor Oligomers (Kirill Shumilov, Alejandra Valderrama-Carvajal, María García-Bonilla, Alicia Rivera)....Pages 109-122 Isolation and Detection of G Protein-Coupled Receptor (GPCR) Heteroreceptor Complexes in Rat Brain Synaptosomal Preparation Using a Combined Brain Subcellular Fractionation/Co-immunoprecipitation (Co-IP) Procedures (Dasiel O. Borroto-Escuela, Manuel Narvaez, Martina Zannoni, Chiara Contri, Minerva Crespo-Ramírez, Michael di Palma et al.)....Pages 123-135 Co-immunoprecipitation of Membrane-Bound Receptors from Subsynaptic Compartments (Wilber Romero-Fernandez, Maria Garcia-Barcelo, Yunis Perez-Betancourt)....Pages 137-145 Coimmunoprecipitation (co-IP) Analysis for Protein-Protein Interactions in the Neurons of the Cerebral Ganglia of the Land Snails of the Genus Polymita During Aestivation (Daily Y. Borroto-Escuela, Idania Hernández-Ramos, Kjell Fuxe, Dasiel O. Borroto-Escuela)....Pages 147-156 Co-immunoprecipitation (Co-IP) of G Protein-Coupled Receptor (GPCR)-Receptor Tyrosine Kinase (RTK) Complexes from the Dorsal Hippocampus of the Rat Brain (Michael Di Palma, Manuel Narvaez, Mariana Pita-Rodríguez, Chiara Contri, Martina Zannoni, Riccardo Cuppini et al.)....Pages 157-164 Back Matter ....Pages 165-166