Hepatic Stem Cells: Methods and Protocols

Description

This volume looks at the liver’s epithelial cells—hepatocytes and cholangiocytes—and their progenitors. This book is divided into five parts: isolation of progenitor cells; characterization of liver progenitors in vivo; generation of hepatocytes, cholangiocytes, and their progenitors; reconstitution of liver tissue structures; and liver injury models. The chapters in this book cover topics such as expansion of bipotential liver stem/progenitor cells (LPCs) from fetal and neonatal liver; identifying progenitor cells involved in liver regeneration in vivo; methods for generating hepatocytes and cholangiocytes from multiple cellular sources; 3D tissue structures ex vivo; and resolving hepatic fibrosis by bone marrow transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Hepatic Stem Cells: Methods and Protocols is a valuable resource to help researchers understand the current theories used to study hepatic stem/progenitor cells, and aid them in performing experiments related to liver biology and pathophysiology. Front Matter ....Pages i-xi Front Matter ....Pages 1-1 Long-Term Culture of Mouse Fetal Hepatic Stem/Progenitor Cells (Atsunori Tsuchiya, Shuji Terai)....Pages 3-8 Isolation of Bipotential Liver Progenitor Cells from Neonatal Mouse Liver (Naoki Tanimizu)....Pages 9-17 Identification and Isolation of Clonogenic Cholangiocyte in Mouse (Bin Li, Craig Dorrell, Pamela S. Canady, Leslie Wakefield)....Pages 19-27 Isolation and Expansion of Rat Hepatocytic Progenitor Cells (Junichi Kino, Norihisa Ichinohe, Masayuki Ishii, Toshihiro Mitaka)....Pages 29-41 Front Matter ....Pages 43-43 Genetic Lineage Tracing of Biliary Epithelial Cells (Teresa Rubio-Tomás, Beatriz Aguilar-Bravo, Pau Sancho-Bru)....Pages 45-57 Specific Labeling and Lineage Tracing of Periportal Hepatocytes Using Two-Step Genetic Recombination (Nicola de Prisco, Eleanor Stout, Joan Font-Burgada)....Pages 59-70 Analysis for the Heterogeneity of Liver Progenitor Cells (Kenji Kamimoto)....Pages 71-82 Chemical Screening Using a Zebrafish Model for Liver Progenitor Cell-Driven Liver Regeneration (Sungjin Ko, Donghun Shin)....Pages 83-90 Front Matter ....Pages 91-91 Conversion of Fibroblasts to Hepatocytes In Vitro (Pengyu Huang, Lulu Sun, Ludi Zhang, Lijian Hui)....Pages 93-101 Conversion of Fibroblasts to Hepatocyte-Like Cells In Vivo (Guangqi Song, Qinggong Yuan, Zhen Dai, Hsin-Chieh Tsay, Xizhong Shen, Michael Ott et al.)....Pages 103-115 Chemically Induced Liver Progenitors (CLiPs): A Novel Cell Source for Hepatocytes and Biliary Epithelial Cells (Takeshi Katsuda, Takahiro Ochiya)....Pages 117-130 Induction of Functional Hepatocytes from Human iPSCs (Taketomo Kido, Yuta Koui)....Pages 131-142 Culture System of Bile Duct-Like Cystic Structures Derived from Human-Inducible Pluripotent Stem Cells (Akihide Kamiya, Kazuya Anzai, Kota Tsuruya, Hiromi Chikada)....Pages 143-153 Front Matter ....Pages 155-155 Generation of Hepatic Tissue Structures Using Multicellular Spheroid Culture (Fumiya Tao, Hirotaka Mihara, Nobuhiko Kojima)....Pages 157-165 Reconstruction of Hepatic Tissue Structures Using Interstitial Flow in a Microfluidic Device (Ryo Sudo)....Pages 167-174 Generation of Hepatic Organoids with Biliary Structures (Takeshi Katsuda, Takahiro Ochiya, Yasuyuki Sakai)....Pages 175-185 Analysis for Remodeling of Hepatic Tissue Structures in 3D During Regeneration (Kota Kaneko)....Pages 187-198 Front Matter ....Pages 199-199 Canine Liver Fibrosis Model to Assess the Functions of Infused Autologous Bone Marrow-Derived Cells (Taro Takami, Kenji Tani, Yasuho Taura, Isao Sakaida)....Pages 201-209 A Rodent Model for Cell Transplantation of Hepatic Progenitor Cells (Sei Kakinuma, Akihide Kamiya)....Pages 211-219 Mouse Model for Hepatocellular Carcinoma and Cholangiocarcinoma Originated from Mature Hepatocytes (Masahiro Yamamoto, Bing Xin, Yuji Nishikawa)....Pages 221-236 Mouse Model for Cholangiocarcinoma from Peribiliary Glands (Hayato Nakagawa, Nobumi Suzuki, Kazuhiko Koike)....Pages 237-245 Back Matter ....Pages 247-248