Stem Cell Niche: Methods and Protocols

Description

This updated collection addresses the intense efforts that have been underway to characterize the cellular-molecular-biochemical elements of not only the hematopoietic stem cell niche but the niches for other stem cells. These specialized microenvironments that regulate stem cell function have continued to inspire tremendous interest as a subject of study in the years since the publication of the first edition. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stem Cell Niche: Methods and Protocols, Second Edition serves as an ideal guide for both experts and novices in the stem cell field. Front Matter ....Pages i-xii In Vitro Maintenance of Multipotent Neural Crest Stem Cells as Crestospheres (Sofie Mohlin, Laura Kerosuo)....Pages 1-11 Analysis of Hematopoietic Niche in the Mouse Embryo (Keai Sinn Tan, Nathalie Brouard, Daisuke Sugiyama)....Pages 13-27 Functional Assays of Stem Cell Properties Derived from Different Niches (Beatriz de Lucas, Laura M. Pérez, Beatriz G. Gálvez)....Pages 29-38 Ex Vivo Visualization and Analysis of the Muscle Stem Cell Niche (Aviva J. Goel, Robert S. Krauss)....Pages 39-50 Stem Cell-Derived Cardiac Spheroids as 3D In Vitro Models of the Human Heart Microenvironment (Madeline Campbell, Mamta Chabria, Gemma A. Figtree, Liudmila Polonchuk, Carmine Gentile)....Pages 51-59 Isolation, Propagation, and Clonogenicity of Intestinal Stem Cells (Prashanthi Ramesh, Aleksandar Buryanov Kirov, David Johannes Huels, Jan Paul Medema)....Pages 61-73 Isolation of Extracellular Vesicles from Subventricular Zone Neural Stem Cells (Mary C. Morton, Victoria N. Neckles, David M. Feliciano)....Pages 75-85 Reconstruction of Regenerative Stem Cell Niche by Cell Aggregate Engineering (Bing-Dong Sui, Bin Zhu, Cheng-Hu Hu, Pan Zhao, Yan Jin)....Pages 87-99 Three-Dimensional Co-culture of Human Hematopoietic Stem/Progenitor Cells and Mesenchymal Stem/Stromal Cells in a Biomimetic Hematopoietic Niche Microenvironment (Marta H. G. Costa, Tiago S. Monteiro, Susana Cardoso, Joaquim M. S. Cabral, Frederico Castelo Ferreira, Cláudia L. da Silva)....Pages 101-119 Investigating the Vascular Niche: Three-Dimensional Co-culture of Human Skeletal Muscle Stem Cells and Endothelial Cells (Claire Latroche, Michèle Weiss-Gayet, Bénédicte Chazaud)....Pages 121-128 Identification and Characterization of Stem Cells in Oral Cancer (Sujit K. Bhutia, Prajna P. Naik, Prakash P. Praharaj, Debasna P. Panigrahi, Chandra S. Bhol, Kewal K. Mahapatra et al.)....Pages 129-139 Determining Competitive Potential of Bone Metastatic Cancer Cells in the Murine Hematopoietic Stem Cell Niche (Sun H. Park, Matthew R. Eber, Russell S. Taichman, Yusuke Shiozawa)....Pages 141-150 Spatial Genomic Analysis: A Multiplexed Transcriptional Profiling Method that Reveals Subpopulations of Cells Within Intact Tissues (Antti Lignell, Laura Kerosuo)....Pages 151-163 3D Culture of Mesenchymal Stem Cells in Alginate Hydrogels (Sílvia J. Bidarra, Cristina C. Barrias)....Pages 165-180 Isolation and Identification of Murine Bone Marrow-Derived Macrophages and Osteomacs from Neonatal and Adult Mice (Joydeep Ghosh, Safa F. Mohamad, Edward F. Srour)....Pages 181-193 Back Matter ....Pages 195-199