ENGLISH

Nitric Oxide Protocols

By Benjamin Hemmens , Bernd Mayer (auth.) , Michael A. Titheradge (eds.)

Book information

Publisher
Humana Press
Year
1998
ISBN
978-0-89603-470-9, 978-1-59259-749-9
DOI
10.1385/1592597491
Language
english
Format
PDF
Filesize
6 MB (6556162 bytes)
Series
Methods in Molecular Biology™ 100
Pages
320\320
Orientation
yes
Scanned
yes
Time added
2013-08-01 04:00:00
Biochemistrygeneral

Description

Michael Titheradge provides both the experienced researcher and the newcomer with time-tested techniques to cover all aspects of NO synthase and metabolism. Topics range from the cloning and expression of the different isoforms of NO synthase to the role of NO in DNA damage and apoptosis and include indirect and direct measurements of NO production, measurement on NO synthase mRNA both in situ and in vitro, and Western blotting and immunohistochemical localization of different isoforms of NO synthase. A variety of alternative techniques are described to enable researchers to set up an assay using either the simplest equipment or the most expensive EPR machines.

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