Nucleic Acid Therapeutics in Cancer

Description

The development of effectively targeted and efficiently delivered nucleic acids promises important advances in the diagnosis and treatment of human malignancies. In Nucleic Acid Therapeutics in Cancer, leading researchers, academic physicians, and clinicians clearly and succinctly review the important issues underlying the therapeutic use of nucleic acid-mediated gene silencing. Topics range from basic methodology and delivery to targeting methods and specific clinical targets. The authors thoroughly explain the latest developments in RNA biology, as well as the underpinnings of RNA interference, oligodeoxynucleotide delivery into cells, and strategies for targeting these molecules to accessible regions within the mRNA. They also provide some examples of how these new therapeutic compounds are being used clinically. Concise yet highly readable, Nucleic Acid Therapeutics in Cancer offers those who require a cutting-edge critical synthesis not only a state-of-the-art account of this promising field, but also an illuminating introduction to their emerging therapeutic uses. Front Matter....Pages i-x Front Matter....Pages 1-1 Antisense Methodology....Pages 3-13 Nucleic Acid Therapeutics....Pages 15-24 Front Matter....Pages 25-25 Targeted Genome Modification Via Triple Helix Formation....Pages 27-43 Therapeutic Applications of Ribozymes....Pages 45-64 Use of Catalytic DNA in Target Validation and Therapeutics....Pages 65-79 Targeted Destruction of Small, Stable RNAs....Pages 81-88 Mechanism of Action of Antisense RNA in Eukaryotic Cells....Pages 89-104 Front Matter....Pages 105-105 The Transport of Oligonucleotides Into Cells....Pages 107-113 Peptide-Mediated Delivery of Antisense Oligonucleotides and Related Material....Pages 115-127 Molecular Vectors for Gene Delivery to Cancer Cells....Pages 129-140 Front Matter....Pages 141-141 Considerations on the Design of Antisense Oligonucleotides....Pages 143-154 Identification of Hybridization Accessible Sequence in Messenger RNA....Pages 155-163 Front Matter....Pages 165-165 Nucleic Acids As Gene-Targeting Therapeutics....Pages 167-176 Y900003 (Isis 3521) and G3139 (Genasense; Oblimersen)....Pages 177-197 Antisense Protein Kinase A-RIα Restores Normal Signal Transduction Signatures to Inhibit Tumor Growth....Pages 199-212 Back Matter....Pages 213-221