ENGLISH

Protein Self-Assembly. Methods and Protocols

By Jennifer J. McManus (ed.)

Book information

Publisher
Springer Science
Year
2019
ISBN
978-1-4939-9677-3, 978-1-4939-9678-0
Language
english
Format
PDF
Filesize
7 MB (7510361 bytes)
Series
Methods in Molecular Biology 2039
Pages
266\261
Topic
Biology Molecular
Time added
2019-07-26 19:06:14

Description

This volume explores experimental and computational approaches to measuring the most widely studied protein assemblies, including condensed liquid phases, aggregates, and crystals. The chapters in this book are organized into three parts: Part One looks at the techniques used to measure protein-protein interactions and equilibrium protein phases in dilute and concentrated protein solutions; Part Two describes methods to measure kinetics of aggregation and to characterize the assembled state; and Part Three details several different computational approaches that are currently used to help researchers understand protein self-assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Protein Self-Assembly: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field. Front Matter ....Pages i-x Front Matter ....Pages 1-1 Measuring Nonspecific Protein–Protein Interactions by Dynamic Light Scattering (Daniel Corbett, Jordan W. Bye, Robin A. Curtis)....Pages 3-21 Light Scattering to Quantify Protein–Protein Interactions at High Protein Concentrations (Mahlet A. Woldeyes, Cesar Calero-Rubio, Eric M. Furst, Christopher J. Roberts)....Pages 23-37 Quantitative Evaluation of Protein Solubility in Aqueous Solutions by PEG-Induced Liquid–Liquid Phase Separation (Ying Wang, Ramil F. Latypov)....Pages 39-49 Measuring Protein Solubility (Neer Asherie)....Pages 51-57 Front Matter ....Pages 59-59 Integral caa3-Cytochrome c Oxidase from Thermus thermophilus: Purification and Crystallization (Orla Slattery, Sabri Cherrak, Tewfik Soulimane)....Pages 61-79 Aggregation Profiling of C9orf72 Dipeptide Repeat Proteins Transgenically Expressed in Drosophila melanogaster Using an Analytical Ultracentrifuge Equipped with Fluorescence Detection (Bashkim Kokona, Nicole R. Cunningham, Jeanne M. Quinn, Robert Fairman)....Pages 81-90 Size Analysis of C9orf72 Dipeptide Repeat Proteins Expressed in Drosophila melanogaster Using Semidenaturing Detergent Agarose Gel Electrophoresis (Nicole R. Cunningham, Bashkim Kokona, Jeanne M. Quinn, Robert Fairman)....Pages 91-101 The Use of High Performance Liquid Chromatography for the Characterization of the Unfolding and Aggregation of Dairy Proteins (Sophie Jeanne Gaspard, André Brodkorb)....Pages 103-115 Differential Scanning Calorimetry to Quantify Heat-Induced Aggregation in Concentrated Protein Solutions (Matthew R. Jacobs, Mark Grace, Alice Blumlein, Jennifer J. McManus)....Pages 117-129 Nanoparticle Tracking Analysis to Examine the Temperature-Induced Aggregation of Proteins (Svenja Sladek, Kate McComiskey, Anne Marie Healy, Lidia Tajber)....Pages 131-139 Evaluation of Temporal Aggregation Processes Using Spatial Intensity Distribution Analysis (Zahra Rattray, Egor Zindy, Kara M. Buzza, Alain Pluen)....Pages 141-155 Fluorescence Correlation Spectroscopy for Particle Sizing in Highly Concentrated Protein Solutions (Judith J. Mittag, Matthew R. Jacobs, Jennifer J. McManus)....Pages 157-171 Size Determination of Protein Oligomers/Aggregates Using Diffusion NMR Spectroscopy (Pancham S. Kandiyal, Ji Yoon Kim, Daniel L. Fortunati, K. H. Mok)....Pages 173-183 Front Matter ....Pages 185-185 Patchy Particle Models to Understand Protein Phase Behavior (Nicoletta Gnan, Francesco Sciortino, Emanuela Zaccarelli)....Pages 187-208 Obtaining Soft Matter Models of Proteins and their Phase Behavior (Irem Altan, Patrick Charbonneau)....Pages 209-227 Binding Free Energies of Conformationally Disordered Peptides Through Extensive Sampling and End-Point Methods (Matthew G. Nixon, Elisa Fadda)....Pages 229-242 Atomistic Simulation Tools to Study Protein Self-Aggregation (Deniz Meneksedag-Erol, Sarah Rauscher)....Pages 243-262 Back Matter ....Pages 263-266

Similar books

Session C11: Ancient Cultural Landscapes in South Europe – their Ecological Setting and Evolution, Session C22: Gardeners from South America, Session S04: Agro-Pastoralism and Early Metallurgy Sessions, Session WS29: The Idea of Enclosure in Recent Iberian Prehistory, Session C88: Rhytmes et causalites des dynamiques de l'anthropisation en Europe entre 6500 ET 500 BC: Hypotheses socio-culturelles et/ou climatiques: Proceedings of the XV UISPP World Congress (Lisbon 4-9 September 2006) / Actes du XV Congrès Mondial (Lisbonne 4-9 Septembre 2006) Vol.36

Session C11: Ancient Cultural Landscapes in South Europe – their Ecological Setting and Evolution, Session C22: Gardeners from South America, Session S04: Agro-Pastoralism and Early Metallurgy Sessions, Session WS29: The Idea of Enclosure in Recent Iberian Prehistory, Session C88: Rhytmes et causalites des dynamiques de l'anthropisation en Europe entre 6500 ET 500 BC: Hypotheses socio-culturelles et/ou climatiques: Proceedings of the XV UISPP World Congress (Lisbon 4-9 September 2006) / Actes du XV Congrès Mondial (Lisbonne 4-9 Septembre 2006) Vol.36

2010 · PDF

THE BRITISH ARMY IN INDIA: ITS PRESERVATION BY AN APPROPRIATE CLOTHING, HOUSING, LOCATING, RECREATIVE EMPLOYMENT, AND HOPEFUL ENCOURAGEMENT OF THE TROOPS. with AN APPENDIX ON INDIA : THE CLIMATE OP ITS HILLS ; THE DEVELOPMENT OF ITS RESODRCBS, INDUSTRY, AND ARTS ; THE ADMINISTRATION OF JUSTICE ; THE BLACK ACT ; THE PROGRESS OF CHRISTIANITY ; THE TRAFFIC IN OPIUM ; THE VALUE OF INDIA ; PERMANENT CAUSES OF DISAFFECTION, AND OF THE RECENT REBELLION ; THE TRADITIONARY POLICY; MISGOVERNMENT BY NATIVE RULERS ; ANNEXATIONS OF THEIR TERRITORY, ETC.

THE BRITISH ARMY IN INDIA: ITS PRESERVATION BY AN APPROPRIATE CLOTHING, HOUSING, LOCATING, RECREATIVE EMPLOYMENT, AND HOPEFUL ENCOURAGEMENT OF THE TROOPS. with AN APPENDIX ON INDIA : THE CLIMATE OP ITS HILLS ; THE DEVELOPMENT OF ITS RESODRCBS, INDUSTRY, AND ARTS ; THE ADMINISTRATION OF JUSTICE ; THE BLACK ACT ; THE PROGRESS OF CHRISTIANITY ; THE TRAFFIC IN OPIUM ; THE VALUE OF INDIA ; PERMANENT CAUSES OF DISAFFECTION, AND OF THE RECENT REBELLION ; THE TRADITIONARY POLICY; MISGOVERNMENT BY NATIVE RULERS ; ANNEXATIONS OF THEIR TERRITORY, ETC.

1858 · PDF

Idries Shah 27 Books Collection : A Perfumed Scorpion, A Veiled Gazelle, Caravan of Dreams, Darkest England, Destination Mecca, Evenings with Idries Shah, Knowing How to Know, Learning How to Learn, Letters and Lectures of Idries Shah, Neglected aspects of Sufi study, Observations, Oriental Magic, Reflections, Seeker after Truth, Special Illumination, Special Problems in the study of Sufi ideas, Sufi thought and action, Tales of the Dervishes, The Dermis Probe, The Elephant in the Dark, The Englishman Handbook, Idries Shah Antology, The Magic Monastery, The natives are restless, wisdom of the Idiots PDF.

Idries Shah 27 Books Collection : A Perfumed Scorpion, A Veiled Gazelle, Caravan of Dreams, Darkest England, Destination Mecca, Evenings with Idries Shah, Knowing How to Know, Learning How to Learn, Letters and Lectures of Idries Shah, Neglected aspects of Sufi study, Observations, Oriental Magic, Reflections, Seeker after Truth, Special Illumination, Special Problems in the study of Sufi ideas, Sufi thought and action, Tales of the Dervishes, The Dermis Probe, The Elephant in the Dark, The Englishman Handbook, Idries Shah Antology, The Magic Monastery, The natives are restless, wisdom of the Idiots PDF.

2022 · PDF

The travels of Capts. Lewis and Clarke from St. Louis, by way of the Missouri and Columbia rivers, to the Pacific ocean; performed in the years 1804, 1805 & 1806, by order of the government of the United States. Containing delineations of the manners, customs, religion, &c. of the Indians, comp. from various authentic sources, and original documents, and a summary of the Statistical view of the Indian nations, from the official communication of Meriwether Lewis. Illustrated with a map of the country, inhabited by the western tribes of Indians

The travels of Capts. Lewis and Clarke from St. Louis, by way of the Missouri and Columbia rivers, to the Pacific ocean; performed in the years 1804, 1805 & 1806, by order of the government of the United States. Containing delineations of the manners, customs, religion, &c. of the Indians, comp. from various authentic sources, and original documents, and a summary of the Statistical view of the Indian nations, from the official communication of Meriwether Lewis. Illustrated with a map of the country, inhabited by the western tribes of Indians

1809 · PDF

Professional Linux kernel architecture ''Wrox programmer to programmer''--Cover. - ''What you are reading right now is the result of an evolution over more than seven years: After two years of writing, the first edition was published in German by Carl Hanser Verlag in 2003. It then described kernel 2.6.0. The test was used as a basis for the low-level design documentation for the EAL4+ security evaluation of Red Hat Enterprise Linux 5, requiring to update it to kernel 2.6.18 (if the EAL acronym does not mean anything to you, then Wikipedia is once more your friend). Hewlett-Packard sponsored the translation into English and has, thankfully, granted the rights to publish the result. Updates to kernel 2.6.24 were then performed specifically for this book''--P. ix

Professional Linux kernel architecture ''Wrox programmer to programmer''--Cover. - ''What you are reading right now is the result of an evolution over more than seven years: After two years of writing, the first edition was published in German by Carl Hanser Verlag in 2003. It then described kernel 2.6.0. The test was used as a basis for the low-level design documentation for the EAL4+ security evaluation of Red Hat Enterprise Linux 5, requiring to update it to kernel 2.6.18 (if the EAL acronym does not mean anything to you, then Wikipedia is once more your friend). Hewlett-Packard sponsored the translation into English and has, thankfully, granted the rights to publish the result. Updates to kernel 2.6.24 were then performed specifically for this book''--P. ix

2008 · PDF