ENGLISH

Decoding the Antibody Repertoire: High Throughput Sequencing of Multiple Transcripts from Single B Cells

By Brandon DeKosky (auth.)

Book information

Publisher
Springer International Publishing
Year
2017
ISBN
978-3-319-58517-8, 978-3-319-58518-5
DOI
10.1007/978-3-319-58518-5
Language
english
Format
PDF
Filesize
3 MB (3439246 bytes)
Series
Springer Theses
Edition
1
Pages
XXVIII, 87\105
Time added
2017-06-25 01:09:09
Antibodies;Genetic Engineering;Human Genetics;Cellgeneral

Description

This thesis outlines the development of the very first technology for high-throughput analysis of paired heavy and light-chain antibody sequences, opening an entirely new window for antibody discovery and the investigation of adaptive immune responses to vaccines and diseases. Previous methods for high-throughput immune repertoire sequencing have been unable to provide information on the identity of immune receptor pairs encoded by individual B or T lymphocytes. The author directly addresses these limitations by designing two new technologies for sequencing multiple mRNA transcripts from up to 10 million isolated, single cells. The techniques developed in this work have enabled comprehensive interrogation of human B-cell repertoires and have been applied for rapid discovery of new human antibodies, to gain new insights into the development of human antibody repertoires, and for analysis of human immune responses to vaccination and disease.

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