ENGLISH

Methods for Affinity-Based Separations of Enzymes and Proteins

By Munishwar N. Gupta , Ipsita Roy (auth.) , Prof. Dr. Munishwar Nath Gupta (eds.)

Book information

Publisher
Birkhäuser Basel
Year
2002
ISBN
978-3-7643-6305-5, 978-3-0348-8127-2
DOI
10.1007/978-3-0348-8127-2
Language
english
Format
PDF
Filesize
17 MB (18284548 bytes)
Series
Methods and Tools in Biosciences and Medicine
Edition
1
Pages
225\234
Time added
2014-01-18 08:00:00
Analytical Chemistry; Biotechnology; Organic Chemigeneral; Cell Biology

Description

One major concern of biotechnology is either using enzymes or producing them. Enzyme/protein production is therefore an important starting point for biotechnology. Bioseparation or Downstream Processing constitutes about 40-90% of the total production cost. Driven by economics, highly selective technologies applicable to large-scale processing have emerged during the last decade. These technologies are slowly diffusing to enzymologists who are working on a smaller scale, looking for fast and efficient purification protocols. The affinity-based techniques (including precipitation, two-phase extractions, expanded bed chromatography, perfusion chromatography and monoliths) described in this volume provide current and new cutting-edge methods. Consequently, the book is of main interest to researchers in biochemistry, biochemical engineering and biotechnology, working either in academic or industrial sectors.

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