ENGLISH

Practical Approach to Mammalian Cell and Organ Culture

By Tapan Kumar Mukherjee (editor) , Parth Malik (editor) , Srirupa Mukherjee (editor)

Book information

Publisher
Springer
Year
2023
ISBN
9811917302, 9789811917301
Language
english
Format
PDF
Filesize
24 MB (25646518 bytes)
Pages
1302\1286
Time added
2024-04-19 16:05:11

Description

This Major Reference Work offers a detailed overview of culturing primary, secondary cell lines, tissues, and organs. It first introduces various types of mammalian cell cultures, infrastructure requirements for a mammalian cell-culture laboratory. The subsequent chapters present the detailed protocols for the isolation of mammalian hematologic organs and cells. It also discusses various cell-based assays for monitoring cell viability, cell proliferation, cytotoxicity, cell senescence, and cell death assays. In addition, the book addresses the various problems encountered while culturing animal cells, their possible causes, and suggested solutions, presenting detailed protocols for isolation and primary culturing of various mammalian cells and hematoimmunologic organs in two dimensions. Lastly, it reviews the various applications of animal-cell culture, stem-cell culture, and tissue and organ culture. As such, this reference book is highly relevant for students and professionals new to cell-culture work as well as to those wishing to expand their skills from cell-line cultures to primary cultures and from conventional 2D cultures to 3D cultures.

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