Vascular Effects of Hydrogen Sulfide: Methods and Protocols

Description

This book explores techniques for exploring hydrogen sulfide (H2S) and its effects on the vascular system through numerous experimental animal models and vascular preparations. Alterations of vascular H2S generation/signaling may be involved in the pathogenesis of systemic and pulmonary arterial hypertension, ischemic heart disease, ischemic stroke, preeclampsia, and erectile dysfunction, and H2S also serves as an attractive target for pharmacotherapy of cardiovascular diseases, as well as possible effects on cancer, wound healing, and diabetic retinopathy, among other pathologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Vascular Effects of Hydrogen Sulfide: Methods and Protocols is an ideal aid for scientists working to extend our knowledge in this valuable and wide-ranging field of study. Front Matter ....Pages i-x Synthesis, Metabolism, and Signaling Mechanisms of Hydrogen Sulfide: An Overview (Jerzy Bełtowski)....Pages 1-8 Lanthionine and Other Relevant Sulfur Amino Acid Metabolites: Detection of Prospective Uremic Toxins in Serum by Multiple Reaction Monitoring Tandem Mass Spectrometry (Alessandra F. Perna, Francesca Pane, Nunzio Sepe, Carolina Fontanarosa, Gabriella Pinto, Miriam Zacchia et al.)....Pages 9-17 Analysis of Vascular Hydrogen Sulfide Biosynthesis (Thomas J. Lechuga, Dong-bao Chen)....Pages 19-36 Measurement of Protein Persulfidation: Improved Tag-Switch Method (Emilia Kouroussis, Bikash Adhikari, Jasmina Zivanovic, Milos R. Filipovic)....Pages 37-50 ProPerDP: A Protein Persulfide Detection Protocol (Éva Dóka, Elias S. J. Arnér, Edward E. Schmidt, Péter Nagy)....Pages 51-77 Vascular Effects of H2S-Donors: Fluorimetric Detection of H2S Generation and Ion Channel Activation in Human Aortic Smooth Muscle Cells (Alma Martelli, Valentina Citi, Vincenzo Calderone)....Pages 79-87 In Vitro Measurement of H2S-Mediated Vasoactive Responses (Sona Cacanyiova, Andrea Berenyiova)....Pages 89-107 In Vivo Measurement of H2S, Polysulfides, and “SSNO− Mix”-Mediated Vasoactive Responses and Evaluation of Ten Hemodynamic Parameters from Rat Arterial Pulse Waveform (Frantisek Kristek, Marian Grman, Karol Ondrias)....Pages 109-124 Simultaneous Measurements of Tension and Free H2S in Mesenteric Arteries (Elise Røge Nielsen, Anna K. Winther, Ulf Simonsen)....Pages 125-136 The Relaxant Mechanisms of Hydrogen Sulfide in Corpus Cavernosum (Fatma Aydinoglu, Nuran Ogulener)....Pages 137-150 Pharmacological Tools for the Study of H2S Contribution to Angiogenesis (Lucia Morbidelli, Martina Monti, Erika Terzuoli)....Pages 151-166 Central Administration of H2S Donors for Studying Cardiovascular Effects of H2S in Rats (Marcin Ufnal, Artur Nowinski)....Pages 167-172 Colonic Delivery of H2S Donors for Studying Cardiovascular Effects of H2S in Rats (Marcin Ufnal, Tomasz Hutsch)....Pages 173-177 Measurements for Sulfide-Mediated Inhibition of Myeloperoxidase Activity (Dorottya Garai, Zoltán Pálinkás, József Balla, Anthony J. Kettle, Péter Nagy)....Pages 179-203 Vascular Myography to Examine Functional Responses of Isolated Blood Vessels (Joanne Hart)....Pages 205-217 Back Matter ....Pages 219-221