ENGLISH

Production of Membrane Proteins: Strategies for Expression and Isolation

By Anne Skaja Robinson

Book information

Publisher
John Wiley & Sons
Year
2011
ISBN
3527327290, 9783527327294
Google Books ID
rMxkd2iG8XsC
Language
english
Format
PDF
Filesize
4 MB (4078563 bytes)
Edition
1
Pages
443\443
Topic
Biology Molecular
Orientation
yes
Scanned
no
Time added
2012-03-09 12:00:00
Биологические дисциплины;Молекулярная биология

Description

Designed as a research-level guide to current strategies and methods of membrane protein production on the small to intermediate scale, this practice-oriented book provides detailed, step-by-step laboratory protocols as well as an explanation of the principles behind each method, together with a discussion of its relative advantages and disadvantages. Following an introductory section on current challenges in membrane protein production, the book goes on to look at expression systems, emerging methods and approaches, and protein specific considerations.Case studies illustrate how to select or sample the optimal production system for any desired membrane protein, saving both time and money on the laboratory as well as the technical production scale.Unique in its coverage of "difficult" proteins with large membrane-embedded domains, proteins from extremophiles, peripheral membrane proteins, and protein fragments.

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