Cyanobacteria: Omics and Manipulation

Description

In this era of the  “Green Planet”,  cyanobacteria are ideally placed for exploitation as  microbial cell factories, both for carbon capture and storage and for the sustainable production of secondary metabolites and biofuels. The application of omics technologies to cyanobacterial research has yielded a wealth of new information. However for today’s busy researchers, trawling through the literature to stay abreast of current developments can be extremely time-consuming. By compiling and summarising the most important topics on cyanobacterial omics and manipulation, the authors of this book provide the reader with a timely overview of the field. Topics covered: The cyanobacterial core-genome with a focus on secondary metabolites; cyanobacterial evolution; genomics of NRPS/PKS biosynthetic gene clusters; RNA-seq based transcriptomic analysis of single cyanobacterial cells; transcriptomics of the responses: genes, sensors, and molecular triggers; transcriptomic and proteomic analysis of diurnal cycles in nitrogen-fixing cyanobacteria; proteomic analysis of post translational modifications; metabolic engineering and systems biology for free fatty acid production; isoprene production; ethanol production:  impact of omics of the model organism Synechocystis on yield enhancement; engineering of alkane production; photoautotrophic polyhydroxyalkanoate production. This cutting-edge text will serve as a valuable resource for all those working in this field and is recommended for all microbiology libraries.   Contents Preface Current Books of Interest 1 The Cyanobacterial Core Genome: Global and Specific Features with a Focus on Secondary Metabolites Introduction Metagenome analysis and information in cyanobacterial research Recent advances in pangenome assignment Detection of core- and clade-specific functions via metagenome analysis General principles of secondary metabolite biosynthesis in cyanobacteria Main classes of secondary metabolites in cyanobacteria Secondary metabolite gene clusters in the cyanobacterial pangenome Conclusions Future trends and challenges 2 Genome-wide Analysis of Cyanobacterial Evolution: The Example of Synechococcus Introduction The origin of Synechococcus The genome and pan-genome of cyanobacteria Speciation in cyanobacteria Genome-scale reconstruction of evolutionary relationships: phylogenomics Conclusion Future trends 3 Genomics of NRPS/PKS Biosynthetic Gene Clusters in Cyanobacteria Introduction Genome mining for NRPS/PKS biosynthetic gene clusters Comparative genomics of biosynthetic gene clusters Biosynthetic gene clusters conservation From prediction to characterization Genomics from isolated strains versus metagenomics Conclusion Future trends 4 RNA-seq Based Transcriptomic Analysis of Single Cyanobacterial Cells Introduction Quantitation of total RNA in single Synechocystis cells Single-cell RNA isolation Whole-transcriptome amplification Quality control RNA-seq analysis of single-cell transcriptome Verification of RNA-seq results Technical and biological significance Conclusions Considerations and future trends 5 Transcriptomics of Cyanobacterial Stress Responses: Genes, Sensors and Molecular Triggers Introduction Heat stress and other abiotic stresses Cold stress and other abiotic stresses Conclusions: Sensors and universal triggers of stress responses 6 Transcriptomic and Proteomic Analysis to Understand Systems-level Properties of Diurnal Cycles in Nitrogen-fixing Cyanobacteria Introduction Systems biology and proteomics Cyanobacterial proteomics Protein–protein interactions Growth of cyanobacteria in the light and dark Conclusion Future research directions 7 Proteomic Analysis of Post Translational Modifications in Cyanobacteria Introduction PTMs and proteomic analysis of PTMs in cyanobacteria Challenge and perspectives in PTM proteomics of cyanobacteria Conclusions 8 Metabolic Engineering and Systems Biology for Free Fatty Acid Production in Cyanobacteria Introduction Metabolic engineering of FFA production in cyanobacteria Systems biology for FFA production in cyanobacteria Conclusions and future trends 9 Terpene Hydrocarbons Production in Cyanobacteria Introduction: state of the art Perspective The process Solar-to-product energy conversion efficiency and photosynthetic productivity Principles of CO2 delivery and terpene hydrocarbons sequestration Efficacy of terpene hydrocarbons as biofuels Efficacy of alcohol molecules as biofuels Conclusion 10 Ethanol Production in Cyanobacteria: Impact of Omics of the Model Organism Synechocystis on Yield Enhancement Introduction Ethanol production in cyanobacteria The construction of the pdc/adh cassette The origin of the pdc/adh gene cassette The Zymomonas mobilis (Zm) pyruvate decarboxylase The native ADH of Synechocystis The Zymomonas mobilis (Zm) ADH The effect of the ADH co-factor Some key issues with metabolic engineering of Synechocystis Strains and substrains and genomics of the cell factory Synechocystis Proteomic analysis in response to ethanol Transcriptomic analysis in response to ethanol Modelling of metabolic flux in Synechocystis to enhance ethanol production Theoretical estimates of ethanol yields Towards industrial production of ethanol from cyanobacteria Conclusion and future trends 11 Engineering of Alkane Production in Cyanobacteria Introduction Alkane profiles in native cyanobacteria Molecular tools for genetic engineering of cyanobacteria Systems biology research on cyanobacterial alkanes Fatty acid production in engineered cyanobacteria Alkane production in engineered cyanobacteria Protein engineering for ADO Conclusions Future trends 12 Photoautotrophic Polyhydroxyalkanoate Production in Cyanobacteria Introduction Cyanobacteria as platforms for biomass production Cyanobacteria are native PHA producers Engineering cyanobacteria for PHA production Future trends Index