Sphingosine-1-Phosphate: Methods and Protocols

Description

This fully updated edition explores methods involving sphingosine-1-phosphate (S1P), a bioactive lysophospholipid which has become the focus of much research interest as it has widespread developmental and physio-pathological actions, controlling events within the nervous, reproductive, gastrointestinal, vascular, respiratory, and immune systems, in addition to having a prominent role in cancer, early mammalian embryogenesis, and stem cells. Here, worldwide experts in the S1P field describe in-depth techniques in an array of cell types and with various physiological applications, showcasing the important effects of S1P in development and in physiopathology. As a volume in the Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the relevant materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and timely, Sphingosine-1-Phosphate: Methods and Protocols, Second Edition is another key resource for scientists working in this fast-moving and dynamic field. Front Matter ....Pages i-x Measuring Sphingosine-1-Phosphate/Protein Interactions with the Kinetic Exclusion Assay (Jonathan K. Fleming, Jonathan M. Wojciak)....Pages 1-8 An Improved Isoform-Selective Assay for Sphingosine Kinase 1 Activity (Melissa R. Pitman, Lorena T. Davies, Stuart M. Pitson)....Pages 9-20 Methods for Analyzing Sphingosine-1-Phosphate Signaling in Human and Mouse Primary Mast Cells (Alena P. Chumanevich, Piper A. Wedman, Carole A. Oskeritzian)....Pages 21-30 Measurement of Lysophosphatidic Acid and Sphingosine-1-Phosphate by Liquid Chromatography-Coupled Electrospray Ionization Tandem Mass Spectrometry (Maria P. Kraemer, Suchismita Halder, Susan S. Smyth, Andrew J. Morris)....Pages 31-42 Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Samples and Cell Lines (Gary M. Reynolds, Barbara Visentin, Roger Sabbadini)....Pages 43-56 A Cleanup Method for Mass Spectrometric Analysis of Sphingosine- and Ceramide-1-Phosphate in Blood and Solid Tissue Using a Phosphate Capture Molecule (Jun-ichi Morishige, Ryouhei Yamashita, Tamotsu Tanaka, Kiyoshi Satouchi)....Pages 57-71 A Rapid Fluorescence Assay for Measuring Sphingosine-1-Phosphate Transporter Activity in Erythrocytes (Naoki Kobayashi, Tsuyoshi Nishi)....Pages 73-82 Analysis of S1P Receptor Expression by Uterine Immune Cells Using Standardized Multi-parametric Flow Cytometry (Jianhong Zhang, Annie Bang, Stephen J. Lye)....Pages 83-97 S1P Synergizes with Wall Shear Stress and Other Angiogenic Factors to Induce Endothelial Cell Sprouting Responses (Camille L. Duran, Roland Kaunas, Kayla J. Bayless)....Pages 99-115 In Vitro Methods to Study the Modulation of Migration and Invasion by Sphingosine-1-Phosphate (Melina G. Castro, Ludmila E. Campos, Yamila I. Rodriguez, Sergio E. Alvarez)....Pages 117-131 Maintenance of Human Embryonic Stem Cells by Sphingosine-1-Phosphate and Platelet-Derived Growth Factor (Raymond C. B. Wong, Martin F. Pera, Alice Pébay)....Pages 133-140 Sphingosine-1-Phosphate (S1P) Signaling in Neural Progenitors (Phillip Callihan, Mohammed Alqinyah, Shelley B. Hooks)....Pages 141-151 Ceramide and S1P Signaling in Embryonic Stem Cell Differentiation (Guanghu Wang, Stefka D. Spassieva, Erhard Bieberich)....Pages 153-171 3D Stacked Construct: A Novel Substitute for Corneal Tissue Engineering (Shrestha Priyadarsini, Sarah E. Nicholas, Dimitrios Karamichos)....Pages 173-180 Back Matter ....Pages 181-182