Bioanalytical Chemistry

Description

Interdisciplinary knowledge is becoming increasingly important to the modern scientist. This invaluable textbook covers bioanalytical chemistry (mainly the analysis of proteins and DNA) and explains everything for the non-biologist. Electrophoresis, mass spectrometry, biosensors, bioassays, DNA and protein sequencing are not necessarily all included in conventional analytical chemistry textbooks. The book describes the basic principles and the applications of instrumental and molecular methods. It is particularly useful to chemistry and engineering students who already have some basic knowledge about analytical chemistry. This revised second edition contains a new chapter on optical spectroscopy, and updated methods and new references throughout. Andreas Manz received the 2015 Inventor Award for "Lifetime Achievement" from the European Patent Office. Petra S Dittrich will be presented with the Heinrich-Emanuel-Merck Award 2015 at EuroAnalysis2015 Conference. Contents Preface To The First Edition Preface To The Second Edition List Of Abbreviations Chapter 1 Biomolecules 1.1 Amino Acids, Peptides and Proteins 1.2 Nucleic Acids 1.3 Biomolecules in Analytical Chemistry References Chapter 2 Chromatography 2.1 The Principle of Chromatography 2.2 Basic Chromatographic Theory 2.3 Liquid Chromatography Techniques for Bioanalysis References Chapter 3 Electrophoresis 3.1 Principle and Theory of Electrophoresis 3.2 Gel Electrophoresis (GE) 3.3 Capillary Electrophoresis (CE) References Chapter 4 Mass Spectrometry 4.1 The Principle of Mass Spectrometry 4.2 Matrix-Assisted Laser Desorption Ionisation — Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) 4.3 Electrospray Ionisation Mass Spectrometry (ESI-MS) References Chapter 5 Optical Spectroscopy 5.1 Absorption of UV and Visible Light 5.1.1 5.2 Fundamentals of Fluorescence Spectroscopy 5.2.1 References Chapter 6 Molecular Recognition In Immunoassays, Biosensors, Dna-arrays And Pyrosequencing 6.1 Bioassays 6.2 Biosensors 6.3 DNA Binding Arrays 6.4 DNA Identification by Pyrosequencing References Chapter 7 Nucleic Acid Analysis 7.1 Extraction and Isolation of Nucleic Acids 7.2 Nucleic Acid Amplification — the Polymerase Chain Reaction (PCR) 7.3 Nucleic Acid Sequencing 7.4 RNA-Sequencing References Chapter 8 Protein Analysis 8.1 Protein Sequencing Strategy 8.2 End-Group Analysis 8.3 Disulfide-Bond Cleavage 8.4 Separation and Molecular Weight Determination of the Subunits 8.5 Amino Acid Composition 8.6 Cleavage of Specific Peptide Bond 8.7 Sequence Determination 8.8 Ordering of Peptide-Fragments 8.9 Determination of Disulfide Bond Positions 8.10 Protein Sequencing by Mass Spectrometry References Index