ENGLISH

siRNA and miRNA Gene Silencing: From Bench to Bedside

By Mouldy Sioud (eds.) , Shigeru Takasaki (auth.)

Book information

Publisher
Humana Press
Year
2009
ISBN
1603275460, 9781603275460
DOI
10.1007/978-1-60327-547-7
Open Library ID
OL24103211M
Language
english
Format
PDF
Filesize
6 MB (6225701 bytes)
Series
Methods in Molecular Biology 487
Edition
1
Pages
472\474
Topic
Biology Molecular
Time added
2011-06-04 13:46:07
Human Genetics; Gene Expression

Description

RNA interference has become a key method in the suppression of gene expression and the development of therapeutic agents, yet there is still the problem of delivery, stability, and the danger of off-target effects such as the silencing of unwanted genes and activation of innate immunity. In siRNA and miRNA Gene Silencing: From Bench to Bedside, expert researchers explore the most recent advances in siRNA design, expression, delivery, in vivo imaging, and methods to minimize siRNA's unwanted effects and promote successful use in patients. As part of the highly successful Methods in Molecular Biology™ series, the chapters focus on their respective subjects with easy-to-use, up-to-date information, including several step-by-step laboratory protocols on topics such as new delivery formulations and strategies with promising applications in vitro and in vivo, validated therapeutic target genes, and components of miRNA function, biogenesis, and interference with virus infection. Comprehensive and cutting-edge, siRNA and miRNA Gene Silencing: From Bench to Bedside offers an excellent collection of chapters to aid all those with an interest in RNAi, gene regulation, and new therapies.

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