Chromosome techniques: a manual

Description

This laboratory manual covers the study of chromosomes in plants, animal and human systems, dealing with the protocols and principles involved. It caters to the requirements of scientists working laboratories, presenting details of the operational mechanism for use at the chromosome level.;1. Pre-Treatment, Hypotonic Treatment, Fixation and Staining 2. Microscopy, Microspectrophotometry, Flow Cytometry, Image Analysis, Confocal Microscopy 3. Study of Banding Patterns of Chromosomes 4. Autoradiography—Light, Fibre and High Resolution 5. Electron Microscopy 6. Study of Chromosomes from Tissue and Protoplast Culture, Cell Fusion and Gene Transfer in Plants 7. Chromosome Analysis following Short- and Long-Term Cultures in Mammalian and Human Systems, from Normal and Malignant Tissues 8. Somatic Cell Fusion in Animals 9. Effects of External Agents and Monitoring for Environmental Toxicants 10. Isolation and Extraction of Nuclei, Chromosomes and Components 11. Micrurgy 12. Identification of Chromosome Segments and Dna Sequences by in Situ Molecular Hybridization 13. Special Molecular Techniques Necessary for Chromosome Analysis 14. Representative Schedules for Direct Observation of Chromosomes from Plants and Animals in Vivo and from Special Materials