Single-Molecule Science: From Super-Resolution Microscopy to DNA Mapping and Diagnostics

Description

Single Molecule Science (SMS) has emerged from developing, using and combining technologies such as super-resolution microscopy, atomic force microscopy, and optical and magnetic tweezers, alongside sophisticated computational and modelling techniques. This comprehensive, edited volume brings together authoritative overviews of these methods from a biological perspective, and highlights how they can be used to observe and track individual molecules and monitor molecular interactions in living cells. Pioneers in this fast-moving field cover topics such as single molecule optical maps, nanomachines, and protein folding and dynamics. A particular emphasis is also given to mapping DNA molecules for diagnostic purposes, and the study of gene expression. With numerous illustrations, this book reveals how SMS has presented us with a new way of understanding life processes. A must-have for researchers and graduate students, as well as those working in industry, primarily in the areas of biophysics, biological imaging, genomics and structural biology. 03.0_pp_iv_iv_Copyright_page 04.0_pp_v_vi_Dedication 05.0_pp_vii_viii_Contents 06.0_pp_ix_xiii_Contributors 07.0_pp_xiv_xviii_Foreword 08.0_pp_xix_xxii_Preface 09.0_pp_1_64_Super-Resolution_Microscopy_and_Molecular_Imaging_Techniques_to_Probe_Biology 09.1_pp_3_19_Introduction_on_Single-Molecule_Science 09.2_pp_20_37_One_Molecule_Two_Molecules_Red_Molecules_Blue_Molecules 09.3_pp_38_48_Multiscale_Fluorescence_Imaging 09.4_pp_49_64_Long-Read_Single-Molecule_Optical_Maps 10.0_pp_65_94_Protein_Folding_Structure_Confirmation_and_Dynamics 10.1_pp_67_79_Single-Molecule_Mechanics_of_Protein_Nanomachines 10.2_pp_80_94_Posttranslational_Protein_Translocation_through_Membranes_at_the_Single-Molecule_Level 11.0_pp_95_124_Mapping_DNA_Molecules_at_the_Single-Molecule_Level 11.1_pp_97_110_Observing_Dynamic_States_of_Single-Molecule_DNA_and_Proteins_Using_Atomic_Force_Microscope 11.2_pp_111_124_Atomic_Force_Microscopy_and_Detecting_a_DNA_Biomarker_of_a_Few_Copies_without_Amplification 12.0_pp_125_141_Single-Molecule_Biology_to_Study_Gene_Expression 12.1_pp_127_141_Single-Molecule_Detection_in_the_Study_of_Gene_Expression 13.0_pp_142_148_Index