ENGLISH

CRISPR: Methods and Protocols

By Magnus Lundgren , Emmanuelle Charpentier , Peter C. Fineran

Book information

Publisher
Humana Press
Year
2015
ISBN
1493926861, 978-1-4939-2686-2, 978-1-4939-2687-9
DOI
10.1007/978-1-4939-2687-9
ISSN
1064-3745
LCC
2015937760
Language
english
Format
PDF
Filesize
10 MB (10819868 bytes)
Series
Methods in Molecular Biology 1311
Edition
1
Pages
xiv+366\381
Topic
Biology\\Genetics
Orientation
yes
Paginated
yes
Scanned
portrait
Time added
2015-05-23 16:30:32
Gene Expression

Description

This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR: Methods and Protocols guides readers through techniques that have been developed specifically for the analysis of CRISPR-Cas and techniques adapted from standard protocols of DNA, RNA and protein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, CRISPR: Methods and Protocols provides a broad list of tools and techniques to study the interdisciplinary aspects of the prokaryotic CRISPR-Cas defense systems.

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