Autoantibodies: Methods and Protocols

Description

This volume covers methods for determination of autoantibodies in rheumatic connective tissue diseases and organ-specific diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Autoantibodies: Methods and Protocols aims to be helpful for all persons working with research and development of autoimmune laboratory diagnostics and for clinicians using autoantibody tests in daily work with patients. Front Matter ....Pages i-xii Autoantibodies as Diagnostic Tools (Gunnar Houen)....Pages 1-11 Nonspecific Binding in Immunoassays for Autoantibodies (Gunnar Houen)....Pages 13-17 Detection of Autoantibodies by Indirect Immunofluorescence Cytochemistry on Hep-2 Cells (Alessandra Dellavance, Luis Eduardo Coelho Andrade)....Pages 19-46 Detection of Anti-neutrophil Cytoplasmic Antibodies (ANCA) by Indirect Immunofluorescence (J. J. B. C. van Beers, J. Vanderlocht, C. Roozendaal, J. Damoiseaux)....Pages 47-62 Determination of Subset-Restricted Anti-neutrophil Cytoplasmic Antibodies (ANCA) by Immunofluorescence Cytochemistry (Firoozeh Amirbeagi, Amanda Welin, Pontus Thulin, Johan Bylund)....Pages 63-77 Determination of Anti-aquaporin 5 Autoantibodies by Immunofluorescence Cytochemistry (Jehan Alam, Sumin Jeon, Youngnim Choi)....Pages 79-87 Determination of cN1A Autoantibodies by Cell-Based Immunofluorescence Cytochemistry (Satoshi Yamashita, Nozomu Tawara)....Pages 89-94 Determination of Agonistically Acting Autoantibodies to the Adrenergic Beta-1 Receptor by Cellular Bioassay (Hanna Davideit, Annekathrin Haberland, Sabine Bartel, Sarah Schulze-Rothe, Johannes Müller, Katrin Wenzel)....Pages 95-102 Determination of Autoantibodies to Salivary Gland Antigens (Li Cui, Xinyuan Zhao, Shen Hu)....Pages 103-112 Measurement of Autoantibodies to Gastric H+,K+-ATPase (ATP4A/B) Using a Luciferase Immunoprecipitation System (LIPS) (Edith Lahner, Ilaria Marzinotto, Cristina Brigatti, Howard Davidson, Janet Wenzlau, Lorenzo Piemonti et al.)....Pages 113-131 Detection of DNA Autoantibodies by Electrophoretic Mobility Shift Assay (Jacqueline Keyhani, Ezzatollah Keyhani)....Pages 133-152 Antigen-Specific Detection of Autoantibodies Against Myeloperoxidase (MPO) and Proteinase 3 (PR3) (J. Vanderlocht, J. J. B. C. van Beers, P. C. Limburg, J. Damoiseaux, C. Roozendaal)....Pages 153-176 Analysis of C3 Nephritic Factors by ELISA (Lillemor Skattum)....Pages 177-182 Analysis of Anti-C1q Autoantibodies by Western Blot (Anci Verlemyr, Lennart Truedsson, Lillemor Skattum)....Pages 183-189 Anti-factor H Autoantibodies Assay by ELISA (Marie Sénant, Marie-Agnes Dragon-Durey)....Pages 191-196 Determination of Autoantibodies to Transglutaminase by Electrochemiluminescence (ECL) Assay (Zhiyuan Zhao, Yong Gu, Dongmei Miao, Eric Hoffmeyer, Yu Liu, Liping Yu)....Pages 197-203 Determination of CRP Autoantibodies by SPR Immunoassay (Qiu-Yu Li, Hai-Yun Li)....Pages 205-219 Histone Protein Epitope Mapping for Autoantibody Recognition in Rheumatoid Arthritis (Feliciana Real-Fernández, Federico Pratesi, Paola Migliorini, Paolo Rovero)....Pages 221-228 Detection of SSA and SSB Antibodies Associated with Primary Sjögren’s Syndrome Using Enzyme-Linked Immunosorbent Assay (Nicole Hartwig Trier)....Pages 229-237 Assessment of Peptidylarginine Deiminase Activity by ELISA Using Human Fibrinogen as Substrate (Dres Damgaard, Claus H. Nielsen)....Pages 239-242 Use of a Citrullinated Peptide Panel for Detection of Anti-Citrullinated Protein Antibodies by Enzyme-Linked Immunosorbent Assay (Nicole Hartwig Trier)....Pages 243-253 Determination of Autoantibodies by Line Immunoblotting (Louise Sternbæk, Tina Friis, Gunnar Houen)....Pages 255-262 Determination of Rheumatoid Factors by ELISA (Nicole Hartwig Trier, Gunnar Houen)....Pages 263-270 Detection of Autoantibodies to Complement Components by Surface Plasmon Resonance-Based Technology (Remi Noe, Sophie Chauvet, Shambhuprasad K. Togarsimalemath, Maria Chiara Marinozzi, Maria Radanova, Vasil V. Vasilev et al.)....Pages 271-280 Back Matter ....Pages 281-289