ENGLISH

Practical Cell Analysis

By Dimitri Pappas(auth.)

Book information

Year
2010
ISBN
9780470741559, 9780470688441
DOI
10.1002/9780470688441
Language
english
Format
PDF
Filesize
10 MB (10446236 bytes)
Pages
306\306
Orientation
yes
Scanned
yes
Time added
2013-08-01 04:00:00

Description

As analytical chemistry and biology move closer together, biologists are performing increasingly sophisticated analytical techniques on cells. Chemists are also turning to cells as a relevant and important sample to study newly developed methods. Practical Cell Analysis provides techniques, hints, and time-saving tips explaining what may be “common knowledge” to one field but are often hidden or unknown to another. Within this practical guide: The procedures and protocols for cell separation, handling cells on a microscope and for using cells in microfluidic devices are presented. Elements of cell culture are taken and combined with the practical advice necessary to maintain a cell lab and to handle cells properly during an analysis The main chapters deal with the fundamentals and applied aspects of each technique, with one complete chapter focusing on statistical considerations of analyzing cells Many diagram-based protocols for some of the more common cell processes are included Chapter summaries and extensive tables are included so that key information can be looked up easily in the lab setting Much like a good manual or cookbook this book is a useful, practical guide and a handy reference for all students, researchers and practitioners involved in cellular analysis. Content: Chapter 1 Getting Started (and Getting the Cells) (pages 1–34): Chapter 2 The Cell?Culture Laboratory (Tools of the Trade) (pages 35–63): Chapter 3 Maintaining Cultures (pages 65–88): Chapter 4 Microscopy of Cells (pages 89–123): Chapter 5 Separating Cells (pages 125–163): Chapter 6 Flow Cytometry: Cell Analysis in the Fast Lane (pages 165–193): Chapter 7 Analyzing Cells with Microfluidic Devices (pages 195–228): Chapter 8 Statistical Considerations (pages 229–246): Chapter 9 Protocols, Probes, and Standards (pages 247–285):

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