Activity-Based Protein Profiling

Description

This volume provides a collection of contemporary perspectives on using activity-based protein profiling (ABPP) for biological discoveries in protein science, microbiology, and immunology. A common theme throughout is the special utility of ABPP to interrogate protein function and small-molecule interactions on a global scale in native biological systems. Each chapter showcases distinct advantages of ABPP applied to diverse protein classes and biological systems. As such, the book offers readers valuable insights into the basic principles of ABPP technology and how to apply this approach to biological questions ranging from the study of post-translational modifications to targeting bacterial effectors in host-pathogen interactions. Front Matter ....Pages i-x Activity-Based Protein Profiling—Enabling Multimodal Functional Studies of Microbial Communities (Christopher Whidbey, Aaron T. Wright)....Pages 1-21 Activity-Based Protein Profiling Methods to Study Bacteria: The Power of Small-Molecule Electrophiles (Shabnam Sharifzadeh, Joshua D. Shirley, Erin E. Carlson)....Pages 23-48 Opportunities and Challenges in Activity-Based Protein Profiling of Mycobacteria (Hiren V. Patel, Michael Li, Jessica C. Seeliger)....Pages 49-72 Activity-Based Protein Profiling at the Host–Pathogen Interface (Yekaterina Kovalyova, Stavroula K. Hatzios)....Pages 73-91 Chemical Proteomic Profiling of Protein Fatty-Acylation in Microbial Pathogens (Tao Peng, Howard C. Hang)....Pages 93-110 How to Target Viral and Bacterial Effector Proteins Interfering with Ubiquitin Signaling (Gerbrand J. van der Heden van Noort, Huib Ovaa)....Pages 111-130 ABPP and Host–Virus Interactions (Geneviève F. Desrochers, John Paul Pezacki)....Pages 131-154 Activity-Based Protein Profiling for the Study of Parasite Biology (Henry J. Benns, Edward W. Tate, Matthew A. Child)....Pages 155-174 Deciphering T Cell Immunometabolism with Activity-Based Protein Profiling (Adam L. Borne, Tao Huang, Rebecca L. McCloud, Boobalan Pachaiyappan, Timothy N. J. Bullock, Ku-Lung Hsu)....Pages 175-210 Small-Molecule Inhibitors of PARPs: From Tools for Investigating ADP-Ribosylation to Therapeutics (Ilsa T. Kirby, Michael S. Cohen)....Pages 211-231 Development of Activity-Based Proteomic Probes for Protein Citrullination (Venkatesh V. Nemmara, Paul R. Thompson)....Pages 233-251 Recent Advances in Activity-Based Protein Profiling of Proteases (Suravi Chakrabarty, Jan Pascal Kahler, Merel A. T. van de Plassche, Roeland Vanhoutte, Steven H. L. Verhelst)....Pages 253-281 Opportunities for Lipid-Based Probes in the Field of Immunology (Sebastiaan T. A. Koenders, Berend Gagestein, Mario van der Stelt)....Pages 283-319 Activity-Based Protein Profiling of Non-ribosomal Peptide Synthetases (Fumihiro Ishikawa, Genzoh Tanabe, Hideaki Kakeya)....Pages 321-349 Target Identification of Bioactive Covalently Acting Natural Products (Daniel K. Nomura, Thomas J. Maimone)....Pages 351-374 Applications of Reactive Cysteine Profiling (Keriann M. Backus)....Pages 375-417