ENGLISH

Mass Spectrometry Data Analysis in Proteomics

By Rune Matthiesen (eds.) , Rune Matthiesen , Kudzai E. Mutenda (auth.)

Book information

Publisher
Humana Press
Year
2007
ISBN
9781588295637, 158829563X
DOI
10.1385/1597452750
Open Library ID
OL17208764M
Language
english
Format
PDF
Filesize
4 MB (4447404 bytes)
Series
Methods in Molecular Biology 367
Edition
1
Pages
320\331
Library
kolxoz-dop
Time added
2010-02-18 13:16:04
Cell Biology; Bioinformatics; Biotechnology; Prote

Description

Mass Spectrometry Data Analysis in Proteomics is an in-depth guide to the theory and practice of analyzing raw mass spectrometry (MS) data in proteomics. As MS is a high throughput technique, proteomic researchers must attend carefully to the associated field of data analysis, and this volume outlines available bioinformatics programs, algorithms, and databases available for MS data analysis. General guidelines for data analysis using search engines such as Mascot, Xtandem, and VEMS are provided, with specific attention to identifying poor quality data and optimizing search parameters. Several different types of MS data are discussed, followed by a description of optimal methods for conversion of raw data into peak lists for input to search engines. Choosing the most accurate and complete databases is emphasized, and a report of available sequence databases is included. Methods for assembling expressed sequence tags (ESTs) into assembled nonredundant databases are provided, along with protocols for further processing the sequences into a format suitable for MS data. Mass Spectrometry Data Analysis in Proteomics describes publicly available applications whenever possible.

Similar books