Guide to Research Techniques in Neuroscience

Description

Neuroscience is, by definition, a multidisciplinary field: some scientists study genes and proteins at the molecular level while others study neural circuitry using electrophysiology and high-resolution optics. A single topic can be studied using techniques from genetics, imaging, biochemistry, or electrophysiology. Therefore, it can be daunting for young scientists or anyone new to neuroscience to learn how to read the primary literature and develop their own experiments. This volume addresses that gap, gathering multidisciplinary knowledge and providing tools for understanding the neuroscience techniques that are essential to the field, and allowing the reader to design experiments in a variety of neuroscience disciplines. Front Cover Guide to Research Techniques in Neuroscience Copyright Contents Foreword to the Second Edition Foreword to the First Edition Preface to the Second Edition Preface to the First Edition Introduction LEVELS OF INVESTIGATION METHODS OF STUDYING THE NERVOUS SYSTEM UNDERSTANDING TECHNIQUES IN NEUROSCIENCE Chapter 1 - Whole-Brain Imaging STRUCTURAL BRAIN IMAGING TECHNIQUES FUNCTIONAL BRAIN IMAGING TECHNIQUES FUNCTIONAL IMAGING EXPERIMENTAL DESIGN AND ANALYSIS CONCLUSION SUGGESTED READING AND REFERENCES Books Review Articles Primary Research Articles—Interesting Examples from the Literature Protocols Websites Chapter 2 - Animal Behavior CONSIDERATIONS FOR CHOOSING AND PERFORMING A BEHAVIORAL ASSAY RODENT BEHAVIORAL PARADIGMS DROSOPHILA BEHAVIORAL PARADIGMS CAENORHABDITIS ELEGANS BEHAVIORAL PARADIGMS NONHUMAN PRIMATE BEHAVIORAL PARADIGMS CONCLUSION SUGGESTED READING AND REFERENCES Books and Review Articles Primary Research Articles—Papers First Describing Standard Behavioral Assays Primary Research Articles—Interesting Examples from the Literature Protocols Chapter 3 - Stereotaxic Surgeries and In Vivo Techniques STEREOTAXIC SURGERIES IMPLANTS FOR LONG-TERM ACCESS TO THE BRAIN MANIPULATING NEURAL ACTIVITY IN VIVO CONCLUSION SUGGESTED READING AND REFERENCES Books Review Articles Primary Research Articles—Interesting Examples from the Literature Protocols Chapter 4 - Electrophysiology A BRIEF REVIEW OF THE ELECTRICAL PROPERTIES OF NEURONS THE ELECTROPHYSIOLOGY RIG CATEGORIES OF ELECTROPHYSIOLOGY RECORDINGS ELECTROPHYSIOLOGY TISSUE PREPARATIONS MANIPULATING NEURAL ACTIVITY DURING ELECTROPHYSIOLOGY EXPERIMENTS CONCLUSION SUGGESTED READING AND REFERENCES Books Review Articles Primary Research Articles—Interesting Examples from the Literature Protocols Chapter 5 - Microscopy ESSENTIAL PRINCIPLES OF MICROSCOPY LIGHT MICROSCOPY FLUORESCENCE MICROSCOPY ELECTRON MICROSCOPY PREPARING AND INTERPRETING MICROSCOPY DATA CONCLUSION SUGGESTED READING AND REFERENCES Books Review Articles Primary Research Articles—Interesting Examples from the Literature Protocols Websites Chapter 6 - Visualizing Neural Structure TISSUE PREPARATION VISUALIZING MORPHOLOGY VISUALIZING GENE AND PROTEIN EXPRESSION VISUALIZING CIRCUITRY CONCLUSION SUGGESTED READING AND REFERENCES Books Review Articles Primary Research Articles—Interesting Examples from the Literature Protocols Websites Chapter 7 - Visualizing Neural Function STATIC MARKERS OF ACTIVITY VISUALIZING NEURAL ACTIVITY VISUALIZING PROTEIN FUNCTION CONCLUSION SUGGESTED READING AND REFERENCES Books Review Articles Primary Research Articles—Interesting Examples from the Literature Protocols Chapter 8 - Manipulating Neural Activity EXPERIMENTAL LOGIC OF MANIPULATING NEURAL ACTIVITY PHYSICAL MANIPULATION ELECTRICAL MANIPULATION PHARMACOLOGICAL MANIPULATION GENETIC MANIPULATION PHARMACOGENETIC MANIPULATION OPTOGENETIC MANIPULATION NEUROMODULATION TECHNIQUES USED IN HUMANS CONCLUSION SUGGESTED READING AND REFERENCES Books Review Articles Primary Research Articles—Interesting Examples from the Literature Protocols Websites Chapter 9 - Identifying Genes and Proteins of Interest A BRIEF REVIEW OF GENES AND PROTEINS GENETIC SCREENS IN SILICO SCREENS MOLECULAR SCREENS CONCLUSION SUGGESTED READING AND REFERENCES Books Review Articles Primary Research Articles—Interesting Examples from the Literature Protocols Websites Chapter 10 - Molecular Cloning and Recombinant DNA Technology ISOLATING DNA FRAGMENTS CLONING DNA IDENTIFYING DNA CONCLUSION SUGGESTED READING AND REFERENCES Books Review Articles Primary Research Articles—Interesting Examples from the Literature Protocols Websites Chapter 11 - Gene Delivery Strategies PHYSICAL GENE DELIVERY CHEMICAL GENE DELIVERY VIRAL GENE DELIVERY CONCLUSION SUGGESTED READING AND REFERENCES Books Review Articles Primary Research Articles—Interesting Examples from the Literature Protocols Websites Chapter 12 - Making and Using Transgenic Organisms TRANSGENES THE TRANSGENIC CONSTRUCT BINARY TRANSGENIC SYSTEMS MAKING TRANSGENIC ORGANISMS CONCLUSION SUGGESTED READING AND REFERENCES Books Review Articles Primary Research Articles—Interesting Examples from the Literature Protocols Websites Chapter 13 - Manipulating Endogenous Genes CLASSICAL GENE TARGETING METHODS NUCLEASE-BASED GENE TARGETING METHODS DISRUPTING GENE PRODUCTS CONCLUSION SUGGESTED READING AND REFERENCES Books Review Articles Primary Research Articles—Interesting Examples from the Literature Protocols Chapter 14 - Cell Culture Techniques CELL CULTURE EQUIPMENT AND REAGENTS IMMORTALIZED CELL LINES PRIMARY CELL AND TISSUE CULTURE STEM CELL CULTURES MANIPULATING CELLS IN CULTURE CONCLUSION SUGGESTED READING AND REFERENCES Books Review Articles Primary Research Articles—Interesting Examples from the Literature Protocols Websites Chapter 15 - Biochemical Assays and Intracellular Signaling INTRODUCTION TO SIGNAL TRANSDUCTION AND INTRACELLULAR SIGNALING FUNDAMENTAL TOOLS USED TO STUDY PROTEINS INVESTIGATING PROTEIN EXPRESSION INVESTIGATING PROTEIN–PROTEIN INTERACTIONS INVESTIGATING POSTTRANSLATIONAL MODIFICATIONS INVESTIGATING PROTEIN–DNA INTERACTIONS CONCLUSION SUGGESTED READING AND REFERENCES Books Review Articles Primary Research Articles—Interesting Examples from the Literature Protocols Websites Glossary Index