Biological Responses to Nanoscale Particles: Molecular and Cellular Aspects and Methodological Approaches

Description

In this book the recent progress accumulated in studies of the interaction of engineered nanoparticles with cells and cellular constituents is presented. The focus is on manufacturing and characterization of nanosized materials, their interactions with biological molecules such as proteins, the mechanisms of transport across biological membranes as well as their effects on biological functions. Fundamental molecular and cellular aspects are in the foreground of the book. A further particularity is the interdisciplinary approach, including fields such as preparatory and analytical chemistry, biophysics and the physics of colloids, advanced microscopy and spectroscopy for in-situ detection of nanoparticles, cellular toxicology and nanomedicine. Nanoscale particles are known to exhibit novel and unprecedented properties that make them different from their corresponding bulk materials. As our ability to control these properties is further advanced, a huge potential to create materials with novel properties and applications emerges. Although the technological and economic benefits of nanomaterials are indisputable, concerns have also been raised that nanoscale structuring of materials might also induce negative health effects. Unfortunately, such negative health effects cannot be deduced from the known toxicity of the corresponding macroscopic material. As a result, there is a major gap in the knowledge necessary for assessing their risk to human health. Front Matter ....Pages i-xx Front Matter ....Pages 1-1 Synthesis of Metallic and Metal Oxide Particles (Kateryna Loza, Matthias Epple)....Pages 3-27 Quantum Dots and Quantum Rods (Christin Rengers, Nikolai Gaponik, Alexander Eychmüller)....Pages 29-51 Polymeric Nanocarriers (Banu Iyisan, Katharina Landfester)....Pages 53-84 Stability of Nanoparticle Dispersions and Particle Agglomeration (Kateryna Loza, Matthias Epple, Michael Maskos)....Pages 85-100 Nanoparticle Behaviour in Complex Media: Methods for Characterizing Physicochemical Properties, Evaluating Protein Corona Formation, and Implications for Biological Studies (Wye-Khay Fong, Thomas L. Moore, Sandor Balog, Dimitri Vanhecke, Laura Rodriguez-Lorenzo, Barbara Rothen-Rutishauser et al.)....Pages 101-150 Front Matter ....Pages 151-151 Nanoparticle-Cell Interactions: Overview of Uptake, Intracellular Fate and Induction of Cell Responses (Barbara Rothen-Rutishauser, Joël Bourquin, Alke Petri-Fink)....Pages 153-170 Cellular and Non-cellular Barriers to Particle Transport Across the Lungs (Nicole Schneider-Daum, Marius Hittinger, Xabier Murgia, Claus-Michael Lehr)....Pages 171-189 Cellular Uptake Mechanisms and Detection of Nanoparticle Uptake by Advanced Imaging Methods (Kleanthis Fytianos, Fabian Blank, Loretta Müller)....Pages 191-211 Imaging Techniques for Probing Nanoparticles in Cells and Skin (Christina Graf, Eckart Rühl)....Pages 213-239 Front Matter ....Pages 241-241 Cellular Defense Mechanisms Following Nanomaterial Exposure: A Focus on Oxidative Stress and Cytotoxicity (Stephen J. Evans, Gareth J. Jenkins, Shareen H. Doak, Martin J. D. Clift)....Pages 243-254 Nanocarriers and Immune Cells (Lorna Moll, Volker Mailänder)....Pages 255-279 Fate and Translocation of (Nano)Particulate Matter in the Gastrointestinal Tract (Andreas Frey, Katrin Ramaker, Niels Röckendorf, Barbara Wollenberg, Ingmar Lautenschläger, Gabriella Gébel et al.)....Pages 281-327 Interactions of Nanoparticles with Skin (Fanny Knorr, Alexa Patzelt, Martina Claudia Meinke, Anika Vogt, Ulrike Blume-Peytavi, Eckart Rühl et al.)....Pages 329-339 Back Matter ....Pages 341-344