Immunotoxicity Testing

Description

This fully updated volume utilizes the expertise of scientists currently engaged in immunotoxicity testing to provide the reader with lab-ready procedures and the background information needed to identify effective testing approaches. Dedicated to identifying and describing exogenous agents that can modify immune function, uncovering modes and mechanisms of action for such agents, and translating data from the laboratory and from the clinic to better predict health risks as well as benefits to those who are exposed to immunomodulatory agents, immunotoxicity testing continues to be a vital field of study, and this collection highlights both the “tried and true” methods as well as alternative protocols that have been more recently developed. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immunotoxicity Testing: Methods and Protocols, Second Edition serves as a valuable contribution to the continued evolution and the application of immunotoxicity testing. Front Matter ....Pages i-xiii Front Matter ....Pages 1-1 Immunotoxicology: A Brief History (Kenneth L. Hastings)....Pages 3-13 Clinical Immunotoxicology (L. Peyton Myers)....Pages 15-26 Investigative Immunotoxicology (Stacey E. Anderson, Hillary L. Shane)....Pages 27-46 Developmental Immunotoxicity (DIT) Testing: Current Recommendations and the Future of DIT Testing (Annalise N. vonderEmbse, Jamie C. DeWitt)....Pages 47-56 Markers of Inflammation (Dori R. Germolec, Kelly A. Shipkowski, Rachel P. Frawley, Ellen Evans)....Pages 57-79 Front Matter ....Pages 81-81 The Sheep Erythrocyte T-Dependent Antibody Response (TDAR) (Gregory S. Ladics)....Pages 83-94 Methylated Bovine Serum Albumin (mBSA)-Induced Delayed-Type Hypersensitivity in Mice (Jessica Margaret Sido)....Pages 95-99 Use of the LLNA:BrdU-ELISA for Skin Sensitization Hazard Assessment (David M. Lehmann)....Pages 101-116 Host Resistance Assays (Stefanie C. M. Burleson, Wendy Jo Freebern, Florence G. Burleson, Gary R. Burleson, Victor J. Johnson, Robert W. Luebke)....Pages 117-145 Enhanced Histopathology Evaluation of Lymphoid Organs (Susan A. Elmore)....Pages 147-168 Tumor Challenges in Immunotoxicity Testing (Sheung P. Ng, Judith T. Zelikoff)....Pages 169-180 Front Matter ....Pages 181-181 Flow Cytometry for the Immunotoxicologist (Scott T. Espenschied, Robert M. Tighe, Kymberly M. Gowdy)....Pages 183-197 Evaluation of Cell-Mediated Immune Function Using the Cytotoxic T-Lymphocyte Assay (Gary R. Burleson, Florence G. Burleson, Rodney R. Dietert)....Pages 199-208 Evaluation of Cell Proliferation and Apoptosis in Immunotoxicity Testing (Mitzi Nagarkatti, Sadiye Amcaoglu Rieder, Prakash S. Nagarkatti)....Pages 209-230 Natural Killer (NK) Cell Assays in Immunotoxicity Testing (Qing Li)....Pages 231-241 Dendritic Cell Assays (Joanna M. Kreitinger, David M. Shepherd)....Pages 243-253 Evaluating Macrophages in Immunotoxicity Testing (Jennifer Franko, Jamie L. McCall, John B. Barnett)....Pages 255-296 Evaluating Cytokines in Immunotoxicity Testing (Emanuela Corsini, Robert V. House)....Pages 297-314 Front Matter ....Pages 315-315 Functional Assays of Hematopoietic Stem Cells in Toxicology Research (Michael D. Laiosa)....Pages 317-333 CD4+ T Cell Differentiation and Activation (Jim Reed, Scott A. Wetzel)....Pages 335-351 Isolation and Identification of Innate Lymphoid Cells (ILCs) for Immunotoxicity Testing (Elia D. Tait Wojno, Celine A. Beamer)....Pages 353-370 Evaluating Antigen-Specific IgE Using the Rat Basophil Leukemia Cell (RBL) Assay (Marsha D. W. Ward, Lisa B. Copeland)....Pages 371-381 Front Matter ....Pages 383-383 Challenges for Integrating Immunotoxicology into the Twenty-First-Century Toxicology Testing Paradigm (Peer W. F. Karmaus, Agnes L. Karmaus)....Pages 385-396 Back Matter ....Pages 397-412