DNA Repair Enzymes: Cell, Molecular, and Chemical Biology

Description

DNA Repair Enzymes, Part A, Volume 591 is the latest volume in the Methods in Enzymology series and the first part of a thematic that focuses on DNA repair enzymes. Topics in this new release include chapters on the Optimization of Native and Formaldehyde iPOND Techniques for Use in Suspension Cells, the Proteomic Analyses of the Eukaryotic Replication Machinery, DNA Fiber Analysis: Mind the Gap!, Comet-FISH for Ultrasensitive Strand-Specific Detection of DNA Damage in Single Cells, Examining DNA Double-Strand Break Repair in a Cell Cycle-Dependent Manner, Base Excision Repair Variants in Cancer, and Fluorescence-Based Reporters for Detection of Mutagenesis in E. coli. Content: Series PagePage ii CopyrightPage iv ContributorsPages xi-xiii PrefacePages xv-xviiiBrandt F. Eichman Chapter One - Optimization of Native and Formaldehyde iPOND Techniques for Use in Suspension CellsPages 1-32Nathaniel E. Wiest, Alan E. Tomkinson Chapter Two - Proteomic Analyses of the Eukaryotic Replication MachineryPages 33-53David Cortez Chapter Three - DNA Fiber Analysis: Mind the Gap!Pages 55-82Annabel Quinet, Denisse Carvajal-Maldonado, Delphine Lemacon, Alessandro Vindigni Chapter Four - Comet-FISH for Ultrasensitive Strand-Specific Detection of DNA Damage in Single CellsPages 83-95Manas Mondal, Jia Guo Chapter Five - Examining DNA Double-Strand Break Repair in a Cell Cycle-Dependent MannerPages 97-118Janapriya Saha, Shih-Ya Wang, Anthony J. Davis Chapter Six - Base Excision Repair Variants in CancerPages 119-157Carolyn G. Marsden, Julie A. Dragon, Susan S. Wallace, Joann B. Sweasy Chapter Seven - Fluorescence-Based Reporters for Detection of Mutagenesis in E. coliPages 159-186Melissa Standley, Jennifer Allen, Layla Cervantes, Joshua Lilly, Manel Camps Chapter Eight - Sequencing DNA for the Oxidatively Modified Base 8-Oxo-7,8-DihydroguaninePages 187-210Aaron M. Fleming, Yun Ding, Cynthia J. Burrows Chapter Nine - Xenopus laevis as Model System to Study DNA Damage Response and Replication Fork StabilityPages 211-232Vincenzo Sannino, Federica Pezzimenti, Stefania Bertora, Vincenzo Costanzo Chapter Ten - Ensemble and Single-Molecule Analysis of Non-Homologous End Joining in Frog Egg ExtractsPages 233-270Thomas G.W. Graham, Johannes C. Walter, Joseph J. Loparo Chapter Eleven - Analysis of Structure-Selective Endonuclease Activities From Yeast and Human ExtractsPages 271-286Joao Matos, Stephen C. West Chapter Twelve - Strategies and Methods of Transcription-Coupled Repair Studies In Vitro and In VivoPages 287-306Vitaly Epshtein, Venu Kamarthapu, Evgeny Nudler Chapter Thirteen - Reconstituted System for the Examination of Repair DNA Synthesis in Homologous RecombinationPages 307-325Youngho Kwon, James M. Daley, Patrick Sung Chapter Fourteen - Current and Emerging Assays for Studying the Primer Synthesis Activities of DNA PrimasesPages 327-353Thomas A. Guilliam, Aidan J. Doherty Chapter Fifteen - Electrical Probes of DNA-Binding ProteinsPages 355-414Jacqueline K. Barton, Phillip L. Bartels, Yingxin Deng, Elizabeth O'Brien Chapter Sixteen - Preparation of Stable Nitrogen Mustard DNA Interstrand Cross-Link Analogs for Biochemical and Cell Biological StudiesPages 415-431Alejandra Castaño, Upasana Roy, Orlando D. Schärer Author IndexPages 433-452 Subject IndexPages 453-460