Gel Electrophoresis - Principles and Basics

Description

InTech, 2012, 366 pages, ISBN: 9535104582 9789535104582In this book, the authors try to present simplified fundamentals of gel-based separation together with exemplarily applications of this versatile technique. The contents of the book crisp and comprehensive, it will receive overwhelming interest and deliver benefits and valuable information to the readers.Contents Basic Principles of Gel Electrophoresis Introduction to Agarose and Polyacrylamide Gel Electrophoresis Matrices with Respect to Their Detection Sensitivities Gel-Electrophoresis and Its Applications Principles of Nucleic Acid Separation by Agarose Gel Electrophoresis Discriminatory Power of Agarose Gel Electrophoresis in DNA Fragments Analysis Gel Electrophoresis of Proteins Gel Electrophoresis of Protein - From Basic Science to Practical Approach Two Dimensional Polyacrylamide Gel Electrophoresis Two-Dimensional Polyacrylamide Gel Electrophoresis - A Practical Perspective High-Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis: ATool for Identification of Polymorphic and Modified Linker Histone Components Two-Dimensional Gel Electrophoresis (2-DE) High Speed Isoelectric Focusing of Proteins Enabling Rapid Two-Dimensional Gel Electrophoresis Denaturing Gradient Gel Electrophoresis (DGGE) Denaturing Gradient Gel Electrophoresis (DGGE) in Microbial Ecology - Insights from Freshwaters Statistical and Bioinformatic Analysis of Electrophoresis Data Statistical Analysis of Gel Electrophoresis Data Quantitative Analysis of Electrophoresis Data - Application to Sequence-Specific Ultrasonic Cleavage of DNA Pulsed Field Gel Electrophoresis (PFGE) The Use of Pulsed Field Gel Electrophoresis in Listeria monocytogenes Sub-Typing - Harmonization at the European Union Level Bacterial Electrophoretic Techniques Electrophoretic Techniques in Microbial Ecology Application of Multiplex PCR, Pulsed-Field Gel Electrophoresis (PFGE), and BOX-PCRfor Molecular Analysis of Enterococci The Use of Pulsed Field Gel Electrophoresis in Listeria monocytogenes Sub-Typing - Comparison with MLVA Method Coupled with Gel Electrophoresis Restriction Fragment Length Polymorphism Analysis of PCR-Amplified Fragments (PCR-RFLP) and Gel Electrophoresis - Valuable Tool for Genotyping and Genetic Fingerprinting Application of Two-Dimensional Gel Electrophoresis to Microbial Systems