Advances in Plant Biotechnology: In Vitro Production of Secondary Metabolites of Industrial Interest (Team-IRA)

Description

The goal of Advances in Plant Biotechnology is to integrate the most recent knowledge on tissue culture, secondary metabolites production under controlled conditions, scaling up to produce them at bioreactor level, and their industrial applications. The biosynthetic pathways and the factors that affect them and the accumulation of metabolites, including metabolomics in medicinal plants, are key components as well. Several extraction and encapsulation technological procedures are reviewed. The structure and function of metabolites from selected commercial crops are reported in detail. Finally, items of paramount importance, such as bioavailability and stability of metabolites in pharma and food products are deeply analyzed. Key Features: Strategies for obtaining selected metabolites through in vitro cultureApplication of biotechnological and bioengineering principles to the management of plant metabolitesDescription of the encapsulation of selected metabolitesBioavailability and stability of metabolites in pharma, food, and industrial sectors This book is mainly addressed to research scientists, technical staff, and private and public organizations involved in plant biotechnology and in its processing industries. Last, but not least, students at all levels and postdoctoral researchers have received special attention from all editors and authors in this publication. Cover Half Title Series Page Title Page Copyright Page Table of Contents Editors Contributors Series Preface Preface Chapter 1 Cell Culture, Somatic Embryogenesis, and Plant Regeneration 1.1 Introduction 1.2 Plant Cell and Tissue Culture for Plant Micropropagation 1.3 Importance of Plant Cell, Tissue, and Organ Culture 1.4 Medicinal Plants and In Vitro Cell Culture 1.5 Bioactive Compounds and the Encapsulation Process 1.6 Conclusions Acknowledgments References Chapter 2 Secondary Metabolites of Mesoamerican Plants Castilleja tenuiflora Benth. and Baccharis conferta Kunth with Anti-inflammatory Effects 2.1 Introduction 2.2 Inflammation 2.3 Mesoamerica as a Source of Plants with Anti-inflammatory Effects 2.4 Current Approaches Toward In-Vitro Production of Anti-inflammatory Compounds 2.5 Castilleja tenuiflora Benth. 2.6 Baccharis conferta Kunth 2.7 Biotechnology of C. tenuiflora and B. conferta 2.8 Conclusions References Chapter 3 Production of Secondary Metabolites by Hairy Root Cultures 3.1 Introduction 3.2 Induction and Establishment of Hairy Root Culture by Agrobacterium rhizogenes Infection 3.3 Effect of the rol Genes of Agrobacterium rhizogenes on the Morphology and Accumulation of Secondary Metabolites in Medicinal Plants 3.4 Plant Regeneration from Hairy Root Cultures 3.5 Agrobacterium rhizogenes as a Biotechnological Tool for Secondary Metabolite Production 3.6 Hairy Root Cultures to Produce Secondary Metabolites with Valuable Biological Activity 3.7 Biotechnological Interventions to Improve Metabolite Production in Hairy Roots 3.8 Synthetic Seeds for Hairy Root Lines Storage 3.9 Large-scale Production of Hairy Roots. 3.10 Conclusions Acknowledgments References Chapter 4 Differentiated Plant Cell Suspension Cultures: The New Promise for Secondary Metabolite Production 4.1 Introduction 4.2 Extraction from Plant Material: Challenging Purifications and Limited Profits 4.3 Semi-synthesis: Chemical and Biological Approaches 4.3.1 Biological Synthesis: Microorganisms 4.4 Plant Suspension Cultures: Classic and Innovative Perspectives 4.4.1 Basics of Suspension Culture Processes 4.5 How to Succeed in Industry: Examples and Limiting Challenges of In Vitro Cultures 4.6 Classic Approach: Secondary Metabolism 4.7 Innovative Approach: Cell Differentiation 4.8 Implementation: Management of Cell Differentiation 4.9 Conclusions Acknowledgements References Chapter 5 Metabolomics in Medicinal Plants 5.1 Introduction 5.2 Metabolome and Plant Metabolomics 5.3 Analytical Techniques and Bioinformatic Tools for Metabolomic Analysis 5.4 Metabolomics Applied to Medicinal Plants 5.5 World Trends on Metabolomics in Medicinal Plants 5.6 Perspectives Acknowledgments References Chapter 6 Extraction, Encapsulation, and Biological Activity of Phenolic Compounds, Alkaloids, and Acetogenins from the Annona Genus 6.1 Introduction 6.2 Traditional Uses of Annona Genus 6.3 Phenolic Compounds, Alkaloids, and Acetogenins from Annona Species 6.4 Extraction and Encapsulation Methods of Phenolic Compounds, Alkaloids, and Acetogenins from Annona Species 6.5 Biological Activities from Extracts of A. muricata, A. cherimola, and A. squamosa 6.5.1 Antioxidant Capacities of Extracts of A. muricata, A. cherimola, and A. squamosa 6.5.2 Cytotoxic Activity from Extracts or Isolated Acetogenins of A. muricata, A. cherimola, and A. squamosa 6.5.3 Anti-Inflammatory Activity from Extracts of A. muricata, A. cherimola, and A. squamosa 6.5.4 Anti-Hyperglycemic Activity from Extracts of A. muricata, A. cherimola, and A. squamosa 6.6 Conclusion Acknowledgments References Chapter 7 Importance of Plant Secondary Metabolites with Biological Activity on Selected Commercial Crops 7.1 Introduction 7.2 Secondary Metabolites Derived from Plants 7.3 Extraction of Secondary Metabolites from Plants 7.4 Diseases Caused by Phytopathogenic Microorganisms in High-value-added Crops 7.5 Secondary Metabolites Used for the Protection of Crops of Commercial Interest 7.6 Secondary Metabolites with Biological Activity Against Phytopathogens: In Vitro Production 7.7 Conclusions and Future Prospects References Chapter 8 Encapsulation of Plant Secondary Metabolites of Industrial Interest 8.1 Introduction 8.2 Overview of Secondary Metabolites 8.2.1 Plant Sources of Secondary Metabolites 8.3 Importance of Secondary Metabolites in Human Health 8.4 Secondary Metabolites Isolation Techniques 8.5 Encapsulation of Secondary Metabolites to Protect Their Biological Activity 8.5.1 Encapsulation of Secondary Metabolites by Nanoemulsion 8.5.2 Encapsulation of Secondary Metabolites by Spray Drying 8.5.3 Encapsulation of Secondary Metabolites by Electrohydrodynamic Processes 8.5.3.1 Encapsulation of Secondary Metabolites by Coaxial Electrospray 8.6 Conclusions References Chapter 9 Bioaccessibility and Stability of Plant Secondary Metabolites in Pharmaceutical and Food Matrices 9.1 Introduction 9.2 Definitions and Methods to Test Bioaccessibility and Stability of Plant Secondary Metabolites 9.3 Bioaccessibility and Stability of Secondary Metabolites in Pharmaceutical Matrices 9.4 Bioaccessibility and Stability of Secondary Metabolites in Food Matrices 9.5 Conclusion References Index