Antibody Expression and Production

Description

Engineered antibodies currently represent over 30% of biopharmaceuticals in clinical trials and their total worldwide sales continue to increase significantly. The importance of antibody applications is reflected in their increasing clinical and industrial applications as well as in the progression of established and emerging production strategies. This volume provides detailed coverage of the generation, optimization, characterization, production and applications of antibody. It provides the necessary theoretical background and description of methods for the expression of antibody in microbial and animal cell cultures and in transgenic animals and plants. There is a strong focus on those issues related to the production of intrabodies, bispecific antibody and antibody fragments and also to novel applications in cancer immunotherapy. Front Matter....Pages i-ix Expression of Antibody in Mammalian Cells....Pages 1-24 Bioreactor Systems for Producing Antibody from Mammalian Cells....Pages 25-52 Production of Antibody in Insect Cells....Pages 53-76 Production of Monoclonal Antibodies in Glycoengineered Pichia pastoris ....Pages 77-98 Production of Antibodies in Hansenula polymorpha ....Pages 99-119 Production of Antibody by Transgenic Avians....Pages 121-141 Production of Antibodies in Plants....Pages 143-164 Production of Antibody Fab Fragments in Escherichia coli ....Pages 165-178 Intrabody Expression in Mammalian Cells....Pages 179-195 Engineering Antibodies for Cancer Therapy....Pages 197-233 Recombinant Bispecific Antibodies for Cancer Therapy....Pages 235-249 The Role of Glycosylation in Therapeutic Antibodies....Pages 251-292 Quality Issues Arising from Post-translational Modification of Recombinant Antibodies....Pages 293-303 Recovery and Purification of Antibody....Pages 305-340 Back Matter....Pages 341-345