Immune Checkpoint Blockade: Methods and Protocols

Description

This detailed volume describes a series of techniques that are essential for evaluating the efficacy of new checkpoint blockade therapies as well as understanding the mechanisms behind the therapies that have already been approved. Beginning with a section on describing the tumor microenvironment and evaluating the immune system at a systemic level, the book continues by covering functional assays that provide answers to questions that may be raised after studying the immune system and its responses to immunotherapies, as well as the use of animal models in this research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immune Checkpoint Blockade: Methods and Protocols serves to aid researchers in furthering our understanding of checkpoint blockage as well as the study of tumor immunology and the development of new immunotherapies. Front Matter ....Pages i-xi Front Matter ....Pages 1-1 Double Immunohistochemistry and Digital Image Analysis (Pablo Moreno-Ruiz, Lina Wik Leiss, Artur Mezheyeuski, Monika Ehnman)....Pages 3-11 Multispectral Fluorescence Imaging Allows for Distinctive Topographic Assessment and Subclassification of Tumor-Infiltrating and Surrounding Immune Cells (Claudia Wickenhauser, Daniel Bethmann, Zipei Feng, Shawn M. Jensen, Carmen Ballesteros-Merino, Chiara Massa et al.)....Pages 13-31 Systems-Level Immune Monitoring by Mass Cytometry (Tadepally Lakshmikanth, Petter Brodin)....Pages 33-48 Immune Monitoring of Cancer Patients by Multi-color Flow Cytometry (Shi Yong Neo, Aine O’Reilly, Yago Pico de Coaña)....Pages 49-65 High-Throughput, Fast, and Sensitive Immunopeptidomics Sample Processing for Mass Spectrometry (Fabio Marino, Chloe Chong, Justine Michaux, Michal Bassani-Sternberg)....Pages 67-79 Identification of Immune Modulatory miRNAs by miRNA Enrichment via RNA Affinity Purification (Uta Sandy Tretbar, Michael Friedrich, Maria-Filothei Lazaridou, Barbara Seliger)....Pages 81-101 Front Matter ....Pages 103-103 Expansion of Tumor-Infiltrating Lymphocytes from Melanoma Tumors (Stina Wickström, Tanja Lövgren)....Pages 105-118 Establishment of Two Dimensional (2D) and Three-Dimensional (3D) Melanoma Primary Cultures as a Tool for In Vitro Drug Resistance Studies (Nataly Cruz Rodríguez, Johanna Lineros, Carol Stefany Rodríguez, Lina María Martínez, Josefa Antonia Rodríguez)....Pages 119-131 Assessment of Antitumor T-Cell Responses by Flow Cytometry After Coculture of Tumor Cells with Autologous Tumor-Infiltrating Lymphocytes (Jeroen Melief, Stina Wickström, Rolf Kiessling, Yago Pico de Coaña)....Pages 133-140 Expansion and Determination of Antigen-Reactive T Cells by Flow Cytometry (Alexander Martens, Graham Pawelec, Christopher Shipp)....Pages 141-151 Assessing Canonical and Adaptive Natural Killer Cell Function in Suppression Assays In Vitro (Dhifaf Sarhan, Jeffrey S. Miller)....Pages 153-166 Evaluating Antibody-Dependent Cell-Mediated Cytotoxicity by Chromium Release Assay (Irene van der Haar Àvila, Patricia Marmol, Rolf Kiessling, Yago Pico de Coaña)....Pages 167-179 Evaluating Antibody-Dependent Cell-Mediated Cytotoxicity by Flow Cytometry (Irene van der Haar Àvila, Patricia Marmol, Jeannette Cany, Rolf Kiessling, Yago Pico de Coaña)....Pages 181-194 Front Matter ....Pages 195-195 Evaluation of Breast Cancer and Melanoma Metastasis in Syngeneic Mouse Models (Kristina Witt, Andreas Lundqvist)....Pages 197-206 Establishment of Melanoma Tumor Xenograft Using Single Cell Line Suspension and Co-injection of Patient-Derived T Cells in Immune-Deficient NSG Mice (Fríða Björk Gunnarsdóttir, Rolf Kiessling, Yago Pico de Coaña)....Pages 207-215 Analysis of Tissue-Resident Immune Cells from Mouse Skin and Lungs by Flow Cytometry (Felipe Galvez-Cancino, Ernesto Lopez, Alvaro Lladser)....Pages 217-222 Back Matter ....Pages 223-225