Cell Culture Technology
Book information
Description
This textbook provides an overview on current cell culture techniques, conditions, and applications specifically focusing on human cell culture. This book is based on lectures, seminars and practical courses in stem cells, tissue engineering, regenerative medicine and 3D cell culture held at the University of Natural Resources and Life Sciences Vienna BOKU and the Gottfried Wilhelm Leibniz University Hannover, complemented by contributions from international experts, and therefore delivers in a compact and clear way important theoretical, as well as practical knowledge to advanced graduate students on cell culture techniques and the current status of research. The book is written for Master students and PhD candidates in biotechnology, tissue engineering and biomedicine working with mammalian, and specifically human cells. It will be of interest to doctoral colleges, Master- and PhD programs teaching courses in this area of research. Front Matter ....Pages I-XII Cell Culture Bioprocess Technology: Biologics and Beyond (Sofie O’Brien, Yonsil Park, Samira Azarin, Wei-Shou Hu)....Pages 1-21 Essentials in Cell Culture (Antonina Lavrentieva)....Pages 23-48 Cell Culture Media (Reinhard Henschler)....Pages 49-59 Establishment of Tumor Cell Lines: From Primary Tumor Cells to a Tumor Cell Line (Katharina Meditz, Beate Rinner)....Pages 61-73 The Third Dimension in Cell Culture: From 2D to 3D Culture Formats (Verena Charwat, Dominik Egger)....Pages 75-90 Biomaterials (Rainer Detsch, Julia Will, Jasmin Hum, Judith A. Roether, Aldo R. Boccaccini)....Pages 91-105 Cell–Surface Interactions (Megan Livingston, F. Kurtis Kasper)....Pages 107-128 Mimicking Physiological Oxygen in Cell Cultures (Nicholas R. Forsyth, Rachel Steeg, Muhammad Ahmad, Mohammed Al Zubaidi, Rakad Al-Jumaily, Marwan Merkhan et al.)....Pages 129-137 Co-cultures (Roland Jacobs, Ralf Hass)....Pages 139-153 Automation of Cell Culture Processes (Jan Hansmann, Ivo Schwedhelm, Andreas Clementi, Dominik Egger, Cornelia Kasper)....Pages 155-168
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