ENGLISH

Basic Confocal Microscopy

By Robert L. Price , W. Gray (Jay) Jerome

Book information

Publisher
Springer International Publishing
Year
2018
ISBN
978-3-319-97453-8, 978-3-319-97454-5
Language
english
Format
PDF
Filesize
21 MB (22470858 bytes)
Edition
2nd ed.
Pages
XI, 368\371
Time added
2019-01-12 07:55:47
Life Sciences; Biological Microscopy; Cell Biology

Description

Basic Confocal Microscopy, Second Edition builds on the successful first edition by keeping the same format and reflecting relevant changes and recent developments in this still-burgeoning field. This format is based on the Confocal Microscopy Workshop that has been taught by several of the authors for nearly 20 years and remains a popular workshop for gaining basic skills in confocal microscopy. While much of the information concerning fluorescence and confocal microscopy that made the first edition a success has not changed in the six years since the book was first published, confocal imaging is an evolving field and recent advances in detector technology, operating software, tissue preparation and clearing, image analysis, and more have been updated to reflect this. Several of these advances are now considered routine in many laboratories, and others such as super resolution techniques built on confocal technology are becoming widely available. Front Matter ....Pages i-xi Introduction and Historical Perspective (Robert L. Price, W. Gray (Jay) Jerome)....Pages 1-20 The Theory of Fluorescence (W. Gray (Jay) Jerome)....Pages 21-36 Fluorescence Microscopy (W. Gray (Jay) Jerome, Robert L. Price)....Pages 37-71 Specimen Preparation (W. Gray (Jay) Jerome, John Fuseler, Caleb A. Padgett, Robert L. Price)....Pages 73-97 Labeling Considerations for Confocal Microscopy (R. M. Albrecht, J. A. Oliver)....Pages 99-134 Digital Imaging (W. Gray (Jay) Jerome)....Pages 135-153 Confocal Digital Image Capture (W. Gray (Jay) Jerome)....Pages 155-186 Types of Confocal Instruments: Basic Principles and Advantages and Disadvantages (John Fuseler, W. Gray (Jay) Jerome, Robert L. Price)....Pages 187-213 Setting the Confocal Microscope Operating Parameters (Amy E. Rowley, Anna M. Harper, Robert L. Price)....Pages 215-277 3D Reconstruction of Confocal Image Data (Thomas C. Trusk)....Pages 279-307 Analysis of Image Similarity and Relationship (Jesse Aaron, Teng-Leong Chew)....Pages 309-333 Ethics and Resources (W. Gray (Jay) Jerome, Robert L. Price)....Pages 335-342 Back Matter ....Pages 343-368

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