ENGLISH

Antibody Engineering: Methods and Protocols

By Benny K. C. Lo

Book information

Publisher
Humana Press
Year
2004
ISBN
9781588290922, 9781592596669, 1588290921
Open Library ID
OL17108197M
Language
english
Format
PDF
Filesize
5 MB (5331371 bytes)
Series
Methods in Molecular Biology
Edition
1
Pages
577\577
Topic
Technique
Scanned
no
Time added
2011-04-20 14:05:05

Description

A core collection of diverse cutting-edge techniques for the generation, expression, optimization, and characterization of recombinant antibodies. Readily reproducible protocols for lead generation range from the cloning of human immunoglobulin genes to the selection and generation of human recombinant antibodies by humanization approaches, molecular display technologies and transgenic animals. Procedures are also described on restructuring antibody leads into monovalent, multivalent, and bispecific binding fragments for a wide variety of in vivo applications. State-of-the-art technologies are described for the characterization of antigen-binding affinity and specificity with novel applications in radioimmunotargeting, cancer immunotherapy, drug abuse, and proteomics.

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