ENGLISH

MERS Coronavirus: Methods and Protocols

By Rahul Vijay

Book information

Publisher
Springer US;Humana
Year
2020
ISBN
978-1-0716-0210-2, 978-1-0716-0211-9
Language
english
Format
PDF
Filesize
5 MB (5634059 bytes)
Series
Methods in Molecular Biology 2099
Edition
1st ed. 2020
Pages
XI, 224\226
Time added
2020-02-08 04:40:55
Biomedicine; Immunology; Virology

Description

This volume provides various techniques and methodologies currently used in the study of MERS-CoV. Chapters are divided into four parts detailing evolution and entry of MERS-coronavirus, genetic alteration and structural determination of MERS-coronavirus proteins, quantitation of virus and anti-viral factors, and mouse models for MERS -coronavirus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, MERS Coronavirus: Methods and Protocols aims to ensure successful results in the further study of this vital field. Front Matter ....Pages i-xi Front Matter ....Pages 1-1 Studying Evolutionary Adaptation of MERS-CoV (Michael Letko, Vincent Munster)....Pages 3-8 Evaluating MERS-CoV Entry Pathways (Enya Qing, Michael P. Hantak, Gautami G. Galpalli, Tom Gallagher)....Pages 9-20 Biochemical Characterization of Middle East Respiratory Syndrome Coronavirus Spike Protein Proteolytic Processing (Gary R. Whittaker, Jean K. Millet)....Pages 21-37 Crystallization and Structural Determination of the Receptor-Binding Domain of MERS-CoV Spike Glycoprotein (Haixia Zhou, Shuyuan Zhang, Xinquan Wang)....Pages 39-50 Front Matter ....Pages 51-51 Bacterial Artificial Chromosome-Based Lambda Red Recombination with the I-SceI Homing Endonuclease for Genetic Alteration of MERS-CoV (Anthony R. Fehr)....Pages 53-68 Deducing the Crystal Structure of MERS-CoV Helicase (Sheng Cui, Wei Hao)....Pages 69-85 Front Matter ....Pages 87-87 Antigen Capture Enzyme-Linked Immunosorbent Assay for Detecting Middle East Respiratory Syndrome Coronavirus in Humans (Joshua Fung, Susanna K. P. Lau, Patrick C. Y. Woo)....Pages 89-97 Quantification of the Middle East Respiratory Syndrome-Coronavirus RNA in Tissues by Quantitative Real-Time RT-PCR (Abdullah Algaissi, Anurodh S. Agrawal, Anwar M. Hashem, Chien-Te K. Tseng)....Pages 99-106 Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralization Assay (Abdullah Algaissi, Anwar M. Hashem)....Pages 107-116 Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera (Abdulrahman Almasaud, Naif Khalaf Alharbi, Anwar M. Hashem)....Pages 117-126 Qualitative and Quantitative Determination of MERS-CoV S1-Specific Antibodies Using ELISA (Sawsan S. Al-amri, Anwar M. Hashem)....Pages 127-133 Front Matter ....Pages 135-135 Genetically Engineering a Susceptible Mouse Model for MERS-CoV-Induced Acute Respiratory Distress Syndrome (Sarah R. Leist, Adam S. Cockrell)....Pages 137-159 Development of a Mouse-Adapted MERS Coronavirus (Kun Li, Paul B. McCray Jr.)....Pages 161-171 Metabolite, Protein, and Lipid Extraction (MPLEx): A Method that Simultaneously Inactivates Middle East Respiratory Syndrome Coronavirus and Allows Analysis of Multiple Host Cell Components Following Infection (Carrie D. Nicora, Amy C. Sims, Kent J. Bloodsworth, Young-Mo Kim, Ronald J. Moore, Jennifer E. Kyle et al.)....Pages 173-194 Evaluation of Activation and Inflammatory Activity of Myeloid Cells During Pathogenic Human Coronavirus Infection (Rudragouda Channappanavar, Stanley Perlman)....Pages 195-204 Histopathologic Evaluation and Scoring of Viral Lung Infection (David K. Meyerholz, Amanda P. Beck)....Pages 205-220 Back Matter ....Pages 221-224

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