Phytochemical Investigations of Genus Terminalia

Description

Genus Terminalia is known to be a rich source of secondary metabolites, mainly polypohenols and triterpenoids. About 39 species have been phytochemically studied leading to the identification of 368 compounds. This work involves the use of hyphenated mass spectrometric methods such as HPLC-ESI-QTOF-MS/MS and UPLC-ESI-QqQLIT-MS/MS for qualitative and quantitative analysis of major bioactive constituents in selected medicinal plants without isolation. It also describes the methods of mass fingerprinting and their use to investigate the plant species variations with the help of statistical software’s (PCA). Markers were identified for quality control and authentications. Cover Half Title Series Page Title Page Copyright Page Table of Contents List of figures List of tables Preface Acknowledgments Authors List of abbreviations and units 1 Terminalia: Ethno- and Phytopharmacological Review 1.1 Introduction 1.2 Traditional Uses and Medicinal Properties 1.3 Phytochemical Constituents 1.4 Pharmacological Activities 1.5 Phytochemical Analysis 2 Identification of Bioactive Phytoconstituents of Terminalia Species by HPLC-ESI-QTOF-MS/MS 2.1 Introduction 2.2 Plant Materials and Chemicals 2.3 Extraction and Sample Preparation 2.4 HPLC-ESI-QTOF-MS/MS Conditions 2.5 Qualitative Analysis of the Phytoconstituents of Terminalia Species 2.5.1 LC-MS Analysis 2.5.2 Identification of Acids 2.5.3 Identification of Flavonoids 2.5.4 Identification of GA Derivatives 2.5.5 Identification of EA Derivatives 2.5.6 Identification of PAs 2.5.6.1 Dimeric B and A-type PAs 2.5.6.2 Trimeric B-type PAs 2.6 Conclusion 3 Quantification of Phenolic Compounds in Six Terminalia Species by UPLC-QqQ[sub(LIT)]-MS/MS 3.1 Introduction 3.2 Plant Materials and Chemicals 3.3 Extraction and Sample Preparation 3.4 UPLC-QqQ[sub(LIT)]-MS Conditions 3.5 Method Development 3.5.1 Analytical Method Validation 3.6 Quantitative Analysis for Phenolics 3.7 Chemometric Analysis 3.8 Conclusion 4 Simultaneous Estimation of Phytoconstituents in T. chebula Fruit and Its Marketed Polyherbal Formulations 4.1 Introduction 4.2 Plant Materials and Chemicals 4.3 Extraction and Sample Preparation 4.4 Quantitative Analysis 4.4.1 Ultra-performance Liquid Chromatography/Triple Quadrupole Linear Ion Trap Mass Spectrometry (UPLC-QqQ[sub(LIT)] -MS) Conditions 4.4.2 Method Development 4.4.3 Analytical Method Validation 4.4.4 Estimation of the 17 Phenolics and Terpenoids in Extracts of T. chebula Fruits and Polyherbal Formulations 4.5 Conclusion References Index