ENGLISH

Golden Gate Cloning: Methods and Protocols

By Daniel Schindler

Book information

Publisher
Humana
Year
2024
ISBN
1071640941, 9781071640944
Language
english
Format
EPUB
Filesize
51 MB (53387895 bytes)
Series
Methods in Molecular Biology; 2850
Pages
517\0
Time added
2024-10-10 07:32:06

Description

This volume provides an overview of the latest protocols that cover the full range of in silico, in vitro, and in vivo approaches to help researchers plan, design, and perform Golden Gate cloning experiments. The chapters in this book cover topics such as Golden Gate cloning of standardized parts; modular DNA construct design for high-throughput Golden Gate Assembly; Golden Gate cloning of Synthetic CRISPR RNA spacer sequences; Golden Gate cloning in Actinobacteria; and YeastFab cloning of toxic genes and protein expression optimization in yeast. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Golden Gate Cloning: Methods and Protocols is a valuable resource for all researchers interested in learning more about this important and developing field. Chapter 3 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

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