ENGLISH

DNA Topoisomerase Protocols: Enzymology & Drugs

By Mary-Ann Bjornsti , Neil Osheroff

Book information

Publisher
Humana Press
Year
2001
ISBN
9780896035126, 9781592590575, 0896035123
LCC
QP624.5.S78 D63 2001
Open Library ID
OL20656175M
Language
english
Format
PDF
Filesize
3 MB (3326952 bytes)
Series
Methods in Molecular Biology
Volume
Part II
Edition
1st
Pages
333\351
Time added
2011-04-20 14:05:05

Description

An unprecedented collection of cutting-edge experimental protocols for investigating the catalytic activities of DNA topoisomerases, as well as their specific interactions with topoisomerase-targeted antitumor and antibacterial drugs. Described by expert experimentalists who have perfected the techniques, these unfailingly reproducible methods include assays for enzyme-catalyzed DNA relaxation/supercoiling, DNA cleavage, DNA nicking, DNA decatenation, and ATP hydrolysis. Several modified DNA substrates, used to dissect enzyme mechanisms by trapping intermediates, are also described. Methodologies to determine the action of topoisomerase-targeted drugs include biochemical assays of drug-induced enzyme-DNA complexes, methods for assaying drug uptake, and cell-based assays for determining the specificity and mechanisms of drug resistance. A companion volume, DNA Topoisomerase Protocols, I: DNA Topology and Enzymes, provides state-of-the-art experimental protocols for investigating DNA structure, topology, and DNA topoisomerase function.

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