ENGLISH

High-Throughput Metabolomics: Methods and Protocols

By Angelo D'Alessandro

Book information

Publisher
Springer New York; Humana
Year
2019
ISBN
978-1-4939-9235-5;978-1-4939-9236-2
Language
english
Format
PDF
Filesize
12 MB (12628821 bytes)
Series
Methods in Molecular Biology 1978
Edition
1st ed.
Pages
XV, 459\452
Time added
2019-09-18 12:17:00
Life Sciences; Metabolomics; Human Genetics; Lipid

Description

This detailed volume focuses on recent technological, computational, and biostatistical advances in the field of high-throughput metabolomics. Chapters encompass methods, platforms, and analytical strategies for steady state measurements and metabolic flux analysis with stable isotope-labeled tracers, in biological matrices of clinical relevance and model organisms. Mass spectrometry-based or orthogonal methods are discussed, along with computational and statistical methods to address data sparsity in high-throughput metabolomics approaches. As a part of the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, High-Throughput Metabolomics: Methods and Protocols provides tools that can bring about the next generation of clinical biochemistry in a cost-effective, rigorous fashion, exponentially advancing our capacity to investigate nature while hastening the advent of personalized medicine. Front Matter ....Pages i-xv Front Matter ....Pages 1-1 Sample Preparation and Reporting Standards for Metabolomics of Adherent Mammalian Cells (Sarah Hayton, Robert D. Trengove, Garth L. Maker)....Pages 3-12 High-Throughput Metabolomics: Isocratic and Gradient Mass Spectrometry-Based Methods (Travis Nemkov, Julie A. Reisz, Sarah Gehrke, Kirk C. Hansen, Angelo D’Alessandro)....Pages 13-26 High-Throughput Metabolomics Based on Direct Mass Spectrometry Analysis in Biomedical Research (Raúl González-Domínguez, Álvaro González-Domínguez, Carmen Segundo, Mónica Schwarz, Ana Sayago, Rosa María Mateos et al.)....Pages 27-38 Traveling Wave Ion Mobility Mass Spectrometry: Metabolomics Applications (Giuseppe Paglia, Giuseppe Astarita)....Pages 39-53 Capillary Electrophoresis Mass Spectrometry as a Tool for Untargeted Metabolomics (Ángeles López-Gonzálvez, Joanna Godzien, Antonia García, Coral Barbas)....Pages 55-77 Front Matter ....Pages 79-79 Overview of Lipid Mass Spectrometry and Lipidomics (Simona Zarini, Robert M. Barkley, Miguel A. Gijón, Robert C. Murphy)....Pages 81-105 LC-MS/MS-MRM-Based Targeted Metabolomics for Quantitative Analysis of Polyunsaturated Fatty Acids and Oxylipins (Xiaoyun Fu, Mikayla Anderson, Yi Wang, James C. Zimring)....Pages 107-120 Untargeted and Semi-targeted Lipid Analysis of Biological Samples Using Mass Spectrometry-Based Metabolomics (Julie A. Reisz, Connie Zheng, Angelo D’Alessandro, Travis Nemkov)....Pages 121-135 HPLC-MS/MS Methods for Diacylglycerol and Sphingolipid Molecular Species in Skeletal Muscle (Kathleen A. Harrison, Bryan C. Bergman)....Pages 137-152 Front Matter ....Pages 153-153 Quantification of d- and l-2-Hydroxyglutarate in Drosophila melanogaster Tissue Samples Using Gas Chromatography-Mass Spectrometry (Hongde Li, Jason M. Tennessen)....Pages 155-165 Comprehensive LC-MS-Based Metabolite Fingerprinting Approach for Plant and Fungal-Derived Samples (Kirstin Feussner, Ivo Feussner)....Pages 167-185 Untargeted Metabolomics of Plant Leaf Tissues (Federica Gevi, Giuseppina Fanelli, Lello Zolla, Sara Rinalducci)....Pages 187-195 Front Matter ....Pages 197-197 Analysis of Arginine Metabolism Using LC-MS and Isotopic Labeling (Gretchen L. Seim, Emily C. Britt, Jing Fan)....Pages 199-217 Quantifying Intermediary Metabolism and Lipogenesis in Cultured Mammalian Cells Using Stable Isotope Tracing and Mass Spectrometry (Thekla Cordes, Christian M. Metallo)....Pages 219-241 Insights into Dynamic Network States Using Metabolomic Data (Reihaneh Mostolizadeh, Andreas Dräger, Neema Jamshidi)....Pages 243-258 Analysis of Endothelial Fatty Acid Metabolism Using Tracer Metabolomics (Joanna Kalucka, Bart Ghesquière, Sarah-Maria Fendt, Peter Carmeliet)....Pages 259-268 Stable Isotope Tracers for Metabolic Pathway Analysis (Sara Violante, Mirela Berisa, Tiffany H. Thomas, Justin R. Cross)....Pages 269-283 Front Matter ....Pages 285-285 Data Processing for GC-MS- and LC-MS-Based Untargeted Metabolomics (Linxing Yao, Amy M. Sheflin, Corey D. Broeckling, Jessica E. Prenni)....Pages 287-299 El-MAVEN: A Fast, Robust, and User-Friendly Mass Spectrometry Data Processing Engine for Metabolomics (Shubhra Agrawal, Sahil Kumar, Raghav Sehgal, Sabu George, Rishabh Gupta, Surbhi Poddar et al.)....Pages 301-321 Pre-analytic Considerations for Mass Spectrometry-Based Untargeted Metabolomics Data (Dominik Reinhold, Harrison Pielke-Lombardo, Sean Jacobson, Debashis Ghosh, Katerina Kechris)....Pages 323-340 Front Matter ....Pages 341-341 Temporal Metabolite, Ion, and Enzyme Activity Profiling Using Fluorescence Microscopy and Genetically Encoded Biosensors (Douglas A. Chapnick, Eric Bunker, Xuedong Liu, William M. Old)....Pages 343-353 Microplate Assays for Spectrophotometric Measurement of Mitochondrial Enzyme Activity (Rachel C. Janssen, Kristen E. Boyle)....Pages 355-368 Quantitative NMR-Based Metabolomics on Tissue Biomarkers and Its Translation into In Vivo Magnetic Resonance Spectroscopy (Natalie J. Serkova, Denise M. Davis, Jenna Steiner, Rajesh Agarwal)....Pages 369-387 Front Matter ....Pages 389-389 Metabolomic Applications in Radiation Biodosimetry (Evagelia C. Laiakis)....Pages 391-402 Metabolomics Analyses to Investigate the Role of Diet and Physical Training (Pol Herrero, Miguel Ángel Rodríguez, Maria Rosa Ras, Antoni del Pino, Lluís Arola, Núria Canela)....Pages 403-430 Blood Biomarkers in Sports Medicine and Performance and the Future of Metabolomics (Iñigo San-Millán)....Pages 431-446 Personalized Metabolomics (David P. Marciano, Michael P. Snyder)....Pages 447-456 Back Matter ....Pages 457-459

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