ENGLISH

Clinical Applications of PCR

By Y. M. Dennis Lo , K. C. Allen Chan (auth.) , Rossa W. K. Chiu , K. C. Allen Chan (eds.)

Book information

Publisher
Humana Press
Year
2006
ISBN
9781588293480, 1588293483
DOI
10.1385/159745074X
LCC
RB43.8.P64 C55 2006
Open Library ID
OL22726790M
Language
english
Format
PDF
Filesize
2 MB (1951861 bytes)
Series
Methods in Molecular Biology 336
Edition
1
Pages
200\211
Library
kolxoz-dop
Time added
2010-02-18 13:16:04
Cell Biology

Description

In this updated second edition of the much praised Clinical Applications of PCR, leading researchers apply molecular diagnostics to the many recent advances that have occurred in polymerase chain reaction (PCR)-based technologies. Highlights include real-time PCR, which allows the technique to be performed in a quantitative manner with improved sensitivity, robustness, and resilience to carryover contamination, mass spectrometric analysis of nucleic acids, and circulating cell-free nucleic acids in plasma. The authors apply these innovations to a broad spectrum of applications, including gene expression, methylation, trace molecule, gene dosage, and single cell analysis. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Clinical Applications of PCR, Second Edition, will help today's laboratorians and researchers working in molecular diagnostics harness the power of these techniques with unprecedented sensitivity and ease.

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