ENGLISH

Quantitative Imaging in Cell Biology

By Jennifer C. Waters and Torsten Wittman (Eds.)

Book information

Publisher
Academic Press
Year
2014
ISBN
9780124202016, 9780124201385
Language
english
Format
PDF
Filesize
46 MB (48723030 bytes)
Series
Methods in Cell Biology Volume 123
Edition
1st Edition
Pages
588 \565
Time added
2017-08-08 21:00:00
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Description

This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Content: Series PagePage ii CopyrightPage iv ContributorsPages xiii-xviii PrefacePages xix-xxJennifer C. Waters, Torsten Wittmann Chapter 1 - Concepts in quantitative fluorescence microscopyPages 1-18Jennifer C. Waters, Torsten Wittmann Chapter 2 - Practical considerations of objective lenses for application in cell biologyPages 19-34Stephen T. Ross, John R. Allen, Michael W. Davidson Chapter 3 - Assessing camera performance for quantitative microscopyPages 35-53Talley J. Lambert, Jennifer C. Waters Chapter 4 - A Practical guide to microscope care and maintenancePages 55-76Lara J. Petrak, Jennifer C. Waters Chapter 5 - Fluorescence live cell imagingPages 77-94Andreas Ettinger, Torsten Wittmann Chapter 6 - Fluorescent proteins for quantitative microscopy: Important properties and practical evaluationPages 95-111Nathan Christopher Shaner Chapter 7 - Quantitative confocal microscopy: Beyond a pretty picturePages 113-134James Jonkman, Claire M. Brown, Richard W. Cole Chapter 8 - Assessing and benchmarking multiphoton microscopes for biologistsPages 135-151Kaitlin Corbin, Henry Pinkard, Sebastian Peck, Peter Beemiller, Matthew F. Krummel Chapter 9 - Spinning-disk confocal microscopy: present technology and future trendsPages 153-175John Oreopoulos, Richard Berman, Mark Browne Chapter 10 - Quantitative deconvolution microscopyPages 177-192Paul C. Goodwin Chapter 11 - Light sheet microscopyPages 193-215Michael Weber, Michaela Mickoleit, Jan Huisken Chapter 12 - DNA curtains: Novel tools for imaging protein–nucleic acid interactions at the single-molecule levelPages 217-234Bridget E. Collins, Ling F. Ye, Daniel Duzdevich, Eric C. Greene Chapter 13 - Nanoscale cellular imaging with scanning angle interference microscopyPages 235-252Christopher DuFort, Matthew Paszek Chapter 14 - Localization microscopy in yeastPages 253-271Markus Mund, Charlotte Kaplan, Jonas Ries Chapter 15 - Imaging cellular ultrastructure by PALM, iPALM, and correlative iPALM-EMPages 273-294Gleb Shtengel, Yilin Wang, Zhen Zhang, Wah Ing Goh, Harald F. Hess, Pakorn Kanchanawong Chapter 16 - Seeing more with structured illumination microscopyPages 295-313Reto Fiolka Chapter 17 - Structured illumination superresolution imaging of the cytoskeletonPages 315-333Ulrike Engel Chapter 18 - Analysis of focal adhesion turnover: A quantitative live-cell imaging examplePages 335-346Samantha J. Stehbens, Torsten Wittmann Chapter 19 - Determining absolute protein numbers by quantitative fluorescence microscopyPages 347-365Jolien Suzanne Verdaasdonk, Josh Lawrimore, Kerry Bloom Chapter 20 - High-Resolution Traction Force MicroscopyPages 367-394Sergey V. Plotnikov, Benedikt Sabass, Ulrich S. Schwarz, Clare M. Waterman Chapter 21 - Experimenters' guide to colocalization studies: Finding a way through indicators and quantifiers, in practicePages 395-408Fabrice P. Cordelières, Susanne Bolte Chapter 22 - User-friendly tools for quantifying the dynamics of cellular morphology and intracellular protein clustersPages 409-427Denis Tsygankov, Pei-Hsuan Chu, Hsin Chen, Timothy C. Elston, Klaus M. Hahn Chapter 23 - Ratiometric Imaging of pH ProbesPages 429-448Bree K. Grillo-Hill, Bradley A. Webb, Diane L. Barber Chapter 24 - Toward quantitative fluorescence microscopy with DNA origami nanorulersPages 449-466Susanne Beater, Mario Raab, Philip Tinnefeld Chapter 25 - Imaging and physically probing kinetochores in live dividing cellsPages 467-487Jonathan Kuhn, Sophie Dumont Chapter 26 - Adaptive fluorescence microscopy by online feedback image analysisPages 489-503Christian Tischer, Volker Hilsenstein, Kirsten Hanson, Rainer Pepperkok Chapter 27 - Open-source solutions for SPIMage processingPages 505-529Christopher Schmied, Evangelia Stamataki, Pavel Tomancak Chapter 28 - Second-harmonic generation imaging of cancerPages 531-546Adib Keikhosravi, Jeremy S. Bredfeldt, Abdul Kader Sagar, Kevin W. Eliceiri IndexPages 547-556 Volume in SeriesPages 557-568

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