Molecular and Cellular Enzymology

Description

Molecular and Cellular Enzymology addresses not only experienced enzymologists but also students, teachers and academic and industrial researchers who are confronted with enzymological problems during their fundamental or applied research. In this field there is an urgent need for training in order to meet the requirements of both research and industrial endeavours. This book consists of several levels. Practical aspects and elementary explanations are given for the benefit of non-specialists’ and students’ understanding. In order to facilitate the task of students, two typographies have been adopted. The main text corresponds to basic knowledge, whereas text in a smaller font provides more specialised information. Specialists will also find topics more deeply expounded with the principal bibliographic references cited. The bibliography, however, is not exhaustive; the choice includes general books and review articles as well as some specialised articles. In this book, for the first time, the different molecular and cellular aspects of enzymology are presented together. Until now, there has been no book available in which these different aspects are treated in the same volume. In addition, besides the theoretical developments, this book provides a wealth of practical information for experimentalists. Front Matter....Pages i-xxi Front Matter....Pages 13-13 General Introduction....Pages 1-12 The Laws of Thermodynamics Concept of Chemical Equilibrium....Pages 15-35 Protein-Ligand Association Equilibria....Pages 37-62 Living Beings, Open Systems....Pages 63-82 Front Matter....Pages 83-83 Chemical Kinetics....Pages 85-101 Kinetics of Enzymatic Reactions with Michaelian Behaviour....Pages 103-191 Experimental Methods to Study Enzymatic Reactions....Pages 193-226 Front Matter....Pages 227-227 Enzyme Origin and Evolution....Pages 229-250 Formation of the Functional Structure of Enzymes: Co- and Post-Translational Events....Pages 251-266 Topology of the Active Centre of Enzymes....Pages 267-340 Front Matter....Pages 341-345 Formation of Enzyme-Substrate Complexes....Pages 347-375 Catalytic Mechanisms....Pages 377-450 Examples of Structure-Function Relationships in Enzymatic Systems....Pages 451-542 Front Matter....Pages 543-545 Regulation by Non-Covalent Interactions....Pages 547-629 Regulation by Covalent Modification....Pages 631-678 Multifunctional Enzymes, Multi-Enzymatic Complexes and Metabolic Channelling....Pages 679-722 Front Matter....Pages 723-724 Localisation and Cellular Compartmentalisation....Pages 727-739 Kinetics of Enzymatic Reactions Catalysed by Immobilised Enzymes....Pages 741-748 Metabolic Control Theory....Pages 749-762 Back Matter....Pages 763-783